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CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants

文献类型： 外文期刊

作者： Cai, Rongxiao 1 ; Chai, Nan 2 ; Zhang, Jiekun 1 ; Tan, Jiantao 3 ; Liu, Yao-Guang 2 ; Zhu, Qinlong 2 ; Zeng, Dongchang 1 ;

作者机构： 1.Guangxi Normal Univ, Univ Engn Res Ctr Bioinformat & Genet Improvement, Key Lab Ecol Rare & Endangered Species & Environm, Guangxi Key Lab Landscape Resources Conservat & Su, Guilin 541006, Guangxi, Peoples R China

2.South China Agr Univ, Coll Agr,State Key Lab Conservat & Utilizat Subtro, Guangdong Basic Res Ctr Excellence Precise Breedin, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

3.Guangdong Acad Agr Sci, Rice Res Inst, Key Lab Genet & Breeding High Qual Rice Southern C, Guangdong Rice Engn Lab, Guangzhou 510640, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2025 年 138 卷 9 期

页码：

收录情况： SCI

摘要： CRISPR/Cas-based genome-editing technology serves as a powerful and versatile tool for genome modification. It has been broadly utilized in crop breeding to enhance traits such as yield, various quality attributes, and biotic and abiotic stress tolerance. Because of public safety concerns over genetically modified organisms (GMOs), many countries have established stringent regulatory policies for genetically modified plants, dramatically limiting the application of related products. However, genome editing in stably transformed plants can result in transgene-free progeny through self-pollination or hybridization or yield DNA-free gene-edited plants via transient transformation. These edited plants materially differ from GMOs and are referred to as genome-edited organisms (GEOs). GEOs have the potential to alleviate regulatory burdens and aid in commercialization. Various methods have been developed to expedite the creation of transgene-free or DNA-free GEOs. This review summarizes the various strategies for creating these types of GEOs based on the CRISPR/Cas systems. It also covers the advantages and drawbacks of these strategies. Additionally, we examine off-target effects and mitigation strategies for plant genome editing and outline regulatory policies for gene-edited crops in selected countries and regions. We hope this review offers valuable references for the advancement of transgene-free and DNA-free GEOs.

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