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Wheat root transcriptional responses against Gaeumannomyces graminis var. tritici

文献类型： 外文期刊

作者： Zhang, Jie 1 ; Yan, Haixia 2 ; Xia, Mingcong 1 ; Han, Xiaoyun 1 ; Xie, Lihua 3 ; Goodwin, Paul H. 4 ; Quan, Xin 1 ; Sun, Runhong 1 ; Wu, Chao 1 ; Yang, Lirong 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Biopesticide Engn Res Ctr, Inst Plant Protect Res, Henan Int Joint Lab Crop Protect, Zhengzhou 450002, Peoples R China

2.Hohhot Environm Monitoring Ctr, Hohhot 010030, Peoples R China

3.Pingdingshan Univ, Sch Chem & Environm Engn, Pingdingshan 467000, Peoples R China

4.Univ Guelph, Sch Environm Sci, Guelph, ON, Canada

关键词： Triticum aestivum L; Gaeumannomyces graminis var; tritici; Wheat roots; RNA-Seq; Differentially expressed genes; Plant defense response

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）

ISSN： 2096-5362

年卷期： 2020 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Wheat root rot caused by Gaeumannomyces graminis var. tritici (Ggt) results in severe yield losses in wheat production worldwide. However, little is known about the molecular mechanism that regulates systemic symptom development in infected wheat. Fluorescent microscopy observation of the stained wheat roots infected by Ggt showed that lesions were visible when the fungus could be detected in the endodermis, pericycle and phloem at 5days post inoculation (dpi), and rust symptoms were visible when there was extensive fungal colonization in the root cortex at 6 dpi. Transcriptome sequencing of Ggt-inoculated wheat roots and healthy control root samples was performed at 5 dpi to identify Ggt-induced gene expression changes in wheat roots at the time of lesion formation. A total of 3973 differentially expressed genes (DEGs) were identified, of which 1004 (25.27%) were up-regulated and 2969 (74.73%) were down-regulated in Ggt-inoculated wheat roots compared with those in control roots. GO annotation and KEGG pathway analysis of these DEGs revealed that many of them were associated with pathogen resistance, such as those involved in oxidation-reduction process, tryptophan biosynthesis process, and phenylpropanoid biosynthesis process. Analysis of DEGs revealed that 15 DEGs were involved in cellular regulation, 57 DEGs in signal transduction pathways, and 75 DEGs in cell wall reorganization, and 23 DEGs are pathogenesis-related proteins. Reverse transcription quantitative PCR (RT-qPCR) of 13 of those DEGs showed that these genes may play roles in wheat resistance against Ggt. Overall, this study represents the first transcriptional profiling of wheat roots in response to Ggt infection and further characterization of DEGs identified in this study may lead to better understanding of resistance against take-all in wheat.

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