Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome
文献类型： 外文期刊
作者： Li, Fengqi 1 ; Wang, Ran 1 ; Qu, Cheng 1 ; Fu, Ningning 1 ; Luo, Chen 1 ; Xu, Yihua 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2016 年 11 卷 8 期
页码：
收录情况： SCI
摘要： The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNAseq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.
- 相关文献
作者其他论文 更多>>
-
Orco mediates olfactory behavior and oviposition in the whitefly Bemisia tabaci
作者：Li, Fengqi;Luo, Chen;Li, Fengqi;Tian, Jiahui;Di, Zhongjuan;Qu, Cheng;Luo, Chen;Fu, Yuejun;Yang, Shiyong
关键词：Whitefly; Orco; Behavior; Host recognition; Oviposition; RNAi
-
Overexpression of CYP6CX4 contributing to field-evolved resistance to flupyradifurone, one novel butenolide insecticide, in Bemisia tabaci from China
作者：Zhang, Qinghe;Tang, Juan;Gao, Bingli;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Wang, Jinda
关键词：Bemisia tabaci; Flupyradifurone; Cytochrome P450
-
Knockdown of one cytochrome P450 gene CYP6DW4 increases the susceptibility of Bemisia tabaci to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide
作者：Tang, Juan;Su, Qi;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran
关键词：Bemisia tabaci; Dimpropyridaz; Insecticide detoxification; Cytochrome P450 monooxygenase
-
Tetraniliprole resistance in field-collected populations of Tuta absoluta (Lepidoptera: Gelechiidae) from China: Baseline susceptibility, cross-resistance, inheritance, and biochemical mechanism
作者：Qu, Cheng;Yao, Jiaqi;Luo, Chen;Wang, Ran;Yao, Jiaqi;Huang, Jianlei;Che, Wunan;Fang, Yong;Fang, Yong
关键词：Tuta absoluta; Tetraniliprole; Cross -resistance; Synergism; Metabolic enzyme; Mode of inheritance
-
Circabidian rhythm of sex pheromone reception in a scarab beetle
作者：Wang, Yinliang;Dong, Huanhuan;Qu, Yafei;Qin, Jianhui;Li, Kebin;Cao, Yazhong;Zhang, Shuai;Yin, Jiao;Wang, Yinliang;Dong, Huanhuan;Zhou, Yuxin;Ren, Bingzhong;Luo, Chen;Leal, Walter S.
关键词：
-
Tetraniliprole risk assessment: Unveiling a hidden threat for managing a generalist herbivore
作者：Qu, Cheng;Li, Yunyi;Zhan, Qianyuan;Luo, Chen;Wang, Ran;Qu, Cheng;Wang, Ran;Wang, Jinda;Guedes, Raul Narciso C.
关键词：Tobacco cutworm; Realized heritability; Baseline of susceptibility; Mode of inheritance; Biochemical mechanism; Transgenerational hormesis
-
Risk assessment, cross-resistance, biochemical mechanism and fitness cost of tetraniliprole resistance in the tomato pinworm Tuta absoluta
作者：Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Yao, Jiaqi;Huang, Jianlei
关键词：Tuta absoluta; Tetraniliprole; Risk assessment; Cross -resistance; Biochemical mechanism; Fitness cost
