您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transformation of Soft Rot Resistant aiiA Gene into Chinese Cabbage (Brassica rapa ssp.pekinensis) by the Pollen-tube Pathway

文献类型： 外文期刊

作者： Han, Li-Wei 1 ; Ge, Yu 2 ; Yao, Lei 3 ; Cui, Chong-Shi 1 ; Qu, Shu-Ping 1 ;

作者机构： 1.Northeast Agr Univ, Dept Hort, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Banana, Haikou Expt Stn, Haikou 570102, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China

关键词： Chinese cabbage;Soft rot;aiiA;Coat protein-mediated resistance;Pollen-tube pathway

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2015 年 17 卷 3 期

页码：

收录情况： SCI

摘要： Bacterial soft rot of Chinese cabbage is the most destructive disease caused by Erwinia carotovora. Until now reliable sources of resistance and effective control methods are unavailable, so development of highly resistant breeding lines is desirable. In the present study, we established an aiiA gene transformation system of soft rot resistant. The aiiA gene was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid vector pBBBast-aiiA (7, 315 kb), which included aiiA gene, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant Bar gene and other elements. Five out of 20632 (0.02%) of T-0 plants was positive for aiiA gene through PCR. Further PCR Southern blot analysis displayed that the gene aiiA segments were transformed into T-0 plants. These transgenic plants could be the potential cultigens against the pathogens of soft rot for Chinese cabbage. (C) 2015 Friends Science Publishers

  • 相关文献

[1]Transformation of Soft Rot Resistant aiiA Gene into Chinese Cabbage (Brassica rapa ssp pekinensis) by the Pollen-tube Pathway. Han, Li-Wei,Cui, Chong-Shi,Qu, Shu-Ping,Ge, Yu,Yao, Lei. 2015

[2]Coat Protein-mediated Resistance to Turnip mosaic virus in Chinese Cabbage (Brassica rapa ssp pekinensis) by the Pollen-tube Pathway. Han, Li Wei,Ge, Yu,Cui, Chong Shi,Qu, Shu Ping,Yao, Lei. 2014

[3]Coat protein-mediated resistance to Turnip mosaic virus in Chinese cabbage (B. rapa ssp pekinensis) by the pollen-tube pathway. Wei, Han Li,Yu, Ge,Shi, Cui Chong,Ping, Qu Shu,Lei, Rao. 2014

[4]Control on soft rot of Chinese cabbage using Harpin protein. Tianlei Qiu,Min Wang,Xiaohong Sun,Meilin Han,Xuming Wang. 2011

[5]Control on soft rot of Chinese cabbage using Harpin protein. Qiu, Tianlei,Wang, Min,Sun, Xiaohong,Han, Meilin,Wang, Xuming. 2011

[6]Erwinia carotovora ssp. Carotovora infection induced "defense lignin" accumulation and lignin biosynthetic gene expression in chinese cabbage (Brassica rapa L. ssp pekinensis). Zhang, Song-He,Yang, Qing,Ma, Rong-Cai. 2007

[7]Analysis for the Global Patent Competition on Chinese Cabbage Molecular Breeding. Zhao, Jingjuan. 2015

[8]Effects of Endogenous Salicylic Acid During Calcium Deficiency-Induced Tipburn in Chinese Cabbage (Brassica rapa L. ssp pekinensis). Su, Tongbing,Yu, Shuancang,Yu, Ruifang,Zhang, Fenglan,Yu, Yangjun,Zhang, Deshuang,Zhao, Xiuyun,Wang, Weihong,Su, Tongbing,Yu, Shuancang,Yu, Ruifang,Zhang, Fenglan,Yu, Yangjun,Zhang, Deshuang,Zhao, Xiuyun,Wang, Weihong.

[9]Expression analysis of major genes involved in signaling pathways during infection of Chinese cabbage with Hyaloperonospora brassicae. Gao, Tingting,Yu, Shuancang,Zhang, Fenglan,Yu, Yangjun,Zhang, Deshuang,Zhao, Xiuyun,Wang, Weihong,Gao, Tingting,Chen, Xuehao. 2014

[10]Proteomic and gene expression analyses during bolting-related leaf color change in Brassica rapa. Zhang, Y. W.,Guo, M. H.,Tang, X. B.,Jin, D.,Zhang, Y. W.,Fang, Z. Y.. 2016

[11]Identification of SCAR markers linked to or, a gene inducing beta-carotene accumulation in Chinese cabbage. Zhang, Fenglan,Wang, Guochen,Wang, Mei,Liu, Xiucun,Zhao, Xiuyun,Yu, Yangjun,Zhang, Deshuang,Yu, Shuancang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台