Coat Protein-mediated Resistance to Turnip mosaic virus in Chinese Cabbage (Brassica rapa ssp pekinensis) by the Pollen-tube Pathway
文献类型： 外文期刊
作者： Han, Li Wei 1 ; Ge, Yu 1 ; Yao, Lei 2 ; Cui, Chong Shi 1 ; Qu, Shu Ping 1 ;
作者机构： 1.Northeast Agr Univ, Dept Hort, Key Lab Biol & Genet Improvement Hort Crops North, Harbin, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing, Peoples R China
关键词： Chinese cabbage (B. rapa ssp pekinensis);Turnip mosaic virus;TuMV-CP;Coat protein-mediated resistance;Pollen-tube pathway
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2014 年 16 卷 6 期
页码：
收录情况： SCI
摘要： Turnip mosaic virus of Chinese cabbage is the most destructive disease. Until now reliable sources of resistance and effective control methods are unavailable, so development of highly resistant breeding lines is desirable. In this research, we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The TuMV-CP gene was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid vector pBBBast-TuMV-CP (9.79 kb), which included TuMV-CP gene, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant Bar gene and other elements. Totally, 0.15% of T-0 plants showed positive for the gene TuMV-CP through PCR. Further PCR Southern blot analysis displayed that the gene TuMV-CP segments were transformed into To plants. (C) 2014 Friends Science Publishers
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