IDENTIFICATION AND CHARACTERIZATION OF 43 NOVEL POLYMORPHIC EST-SSR MARKERS FOR ARUM LILY, ZANTEDESCHIA AETHIOPICA (ARACEAE)
文献类型： 外文期刊
作者： Wei, Zun-Zheng 1 ; Luo, Lai-Bo 2 ; Zhang, Hua-Li 3 ; Xiong, Min 1 ; Wang, Xian 1 ; Zhou, Di 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Guiyang Med Coll, Sch Basic Med Coll, Guiyang 550004, Guizhou, Peoples R China
3.Beijing Inst Landscape & Garden, Beijing 100102, Peoples R China
关键词： Araceae;arum lily;expressed sequence tags (ESTs);simple sequence repeat (SSR) markers;Zantedeschia aethiopica
期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）
ISSN： 0002-9122
年卷期： 2012 年 99 卷 12 期
页码：
收录情况： SCI
摘要： Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. Methods and Results: Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. Conclusions: These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.
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