Development and bioassay of transgenic Chinese cabbage expressing potato proteinase inhibitor II gene
文献类型： 外文期刊
作者： Zhang, Junjie 1 ; Liu, Fan 1 ; Yao, Lei 1 ; Luo, Chen 4 ; Yin, Yue 1 ; Wang, Guixiang 1 ; Huang, Yubi 3 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China
3.Sichuan Agr Univ, Coll Agron, Yaan 625014, Sichuan, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Plant Protect & Environm Protect Inst, Beijing 100097, Peoples R China
关键词： transgenic Chinese cabbage;Pieris rapae;Plutella xylostella;insect resistance;proteinase inhibitor gene II (pinII);chimera
期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）
ISSN： 1344-7610
年卷期： 2012 年 62 卷 2 期
页码：
收录情况： SCI
摘要： Lepidopteran larvae are the most injurious pests of Chinese cabbage production. We attempted the development of transgenic Chinese cabbage expressing the potato proteinase inhibitor II gene (pinII) and bioassayed the pest-repelling ability of these transgenic plants. Cotyledons with petioles from aseptic seedlings were used as explants for Agrobacterium-mediated in vitro transformation. Agrobacterium tumefaciens C58 contained the binary vector pBBBasta-pinII-bar comprising pial and bar genes. Plants showing vigorous PPT resistance were obtained by a series concentration selection for PPT resistance and subsequent regeneration of leaf explants dissected from the putative chimera. Transgenic plants were confirmed by PCR and genomic Southern blotting, which showed that the bar and pinII genes were integrated into the plant genome. Double haploid homozygous transgenic plants were obtained by microspore culture. The pinII expression was detected using quantitative real time polymerase chain reaction (qRT-PCR) and detection of PINII protein content in the transgenic homozygous lines. Insect-feeding trials using the larvae of cabbage worm (Pieris rapae) and the larvae of the diamondback moth (Plutella xylostella) showed higher larval mortality, stunted larval development, and lower pupal weights, pupation rates, and eclosion rates in most of the transgenic lines in comparison with the corresponding values in the non-transformed wild-type line.
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