您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterization of novel TaNAC genes in wheat and overexpression of TaNAC2a confers drought tolerance in tobacco

文献类型： 外文期刊

作者： Tang, YiMiao 2 ; Liu, MeiYing 1 ; Gao, ShiQing 2 ; Zhang, Zhao 1 ; Zhao, Xin 2 ; Zhao, ChangPing 2 ; Zhang, FengTing 2 ;

作者机构： 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：4.5； 五年影响因子：4.576 ）

ISSN： 0031-9317

年卷期： 2012 年 144 卷 3 期

页码：

收录情况： SCI

摘要： Plant-specific NAC (NAM/ATAF/CUC) transcription factors (TFs) have been reported to play a role in diverse stress responses and developmental processes. We show here that six new genes encoding NAC TFs in wheat (Triticum aestivum) were identified (named as TaNAC2a, TaNAC4a, TaNAC6, TaNAC7, TaNAC13 and TaNTL5, respectively), and we classified them into three groups: stress-related NACs, development-related NACs and NTLs (membrane-associated TFs belonging to NAC) by phylogenetic analysis. All TaNACs were induced by one or several kinds of stress treatments including dehydration, salinity and low temperature, whereas different genes showed different expression levels. All these TaNACs, except TaNAC7, were proven to have transcriptional activation activity in the yeast strain AH109 by transactivation analysis. Furthermore, subcellular localization analysis revealed that four TaNAC:GFP (green fluorescent protein) fusion proteins were localized in the nucleus, TaNAC2a:GFP mainly located in the nucleus and the plasma membrane, TaNTL5:GFP was associated with the membrane, while truncated TaNTL5(?TM):GFP (lacking the transmembrane motif) was detected exclusively in the nucleus. Semi-quantitative reverse transcription polymerase chain reaction analysis demonstrated that five genes exhibited organ-specific expression. Transgenic tobacco plants overexpressing TaNAC2a showed higher fresh weight and dry weight than non-transgenic plants under drought condition, which indicated that the transgene improved tobacco tolerance to drought treatment. Together, these results provided a preliminary characterization of six TaNACs, which possessed a potential role in improving stress tolerance and the regulation of development in wheat, and suggested that TaNAC2a was potentially useful for engineering drought tolerant plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台