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Optimization of butanol production from corn straw hydrolysate by Clostridium acetobutylicum using response surface method

文献类型： 外文期刊

作者： Lin YouSheng 1 ; Wang Jing 2 ; Wang XuMing 1 ; Sun XiaoHong 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China

2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China

关键词： enzymatic hydrolysis;butanol fermentation;Plackett-Burman design;response surface method

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2011 年 56 卷 14 期

页码：

收录情况： SCI

摘要： Butanol is a new kind of very potential biofuels. Enzymatic hydrolysis of corn stalk was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8008. Plackett-Burman (P-B) design and Central Composite Design (CCD) were adopted to screen crucial factors during fermentation as well as the optimization of experimental conditions. The result demonstrated that among the seven factors, namely, Yeast extract, (NH4)(2)SO4, KH2PO4, MgSO4, FeSO4, CuSO4 and CaCO3, only CaCO3 was selected as the most critical factor. The optimization experiment results for CaCO3 usage, temperature and reaction time by CCD were determined to be 5.04 g/L, 35A degrees C and 70 h, respectively. A corresponding mathematical model was established to predict the fermentation experiment and maximum butanol yield of 6.57 g/L was acquired. The result of verification experiment under the optimum conditions showed that 6.20 g/L was the maximum butanol yield. This demonstrated that statistical method was a powerful tool for the optimization of butanol production from enzymatic hydrolysis of corn stalk.

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