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Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties

文献类型： 外文期刊

作者： Wang, Feng-Ge 1 ; Tian, Hong-Li 1 ; Zhao, Jiu-Ran 1 ; Yi, Hong-Mei 1 ; Wang, Lu 1 ; Song, Wei 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100097, Peoples R China

关键词： Maize;SSR;core primers;DNA fingerprinting

期刊名称：MAYDICA （ 影响因子：0.542； 五年影响因子：0.605 ）

ISSN： 0025-6153

年卷期： 2011 年 56 卷 1 期

页码：

收录情况： SCI

摘要： A core set of 60 SSRs was selected and modified using 231 Chinese and USA maize (Zea mays L) inbred lines from more than 2000 SSRs for DNA fingerprinting analysis. All 60 SSR markers met the following criteria: (1) amplification of a single locus; (2) distinct amplification products; (3) adequate intervals between adjacent alleles; (4) suitable PCR fragment size; (5) reasonable discrimination power (DP); and (6) even distribution across the maize genome. Furthermore, the 60 SSR primers were re-designed to adjust the PCR product size. Together with the application of four different fluorescent dyes, a high-throughput 10-plex capillary electrophoresis platform was explored. The 60 core SSR markers were further divided into three groups (20 SSRs per group) according to peak morphology and DP value. Groups I, II and III were used in DNA fingerprinting analysis as a basic core, an expanded core and a candidate core set respectively. The allele number per locus varied from three to 22 with an average of 8.95; the average number of alleles per group I, II and III was a respective 7.35, 7.8 and 11.4. The DP values ranged from 0.366 to 0.913, with an average of 0.718 among all loci; the average group DP values were 0.697, 0.718 and 0.737 for groups I, II and III respectively; and the cumulative values of discrimination power (CDP) approached 1 for all groups. Cluster analysis results using 60 selected loci divided the Chinese inbred lines into six groups, including Luda Red Cob, P, Improved Reid, Tang-si-ping-tou, Waxy and Lancaster. The USA inbred lines were segregated into four groups, including SSS, Lancaster, lodent and Oh43/Oh07Mid mixed.

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