Fermentative production of Harpin protein by a recombinant E. coli strain and its toxicity determination
文献类型： 外文期刊
作者： Wang, Min 1 ; Sun, Xiaohong 1 ; Qiu, Tianlei 1 ; Han, Meilin 1 ; Wang, Xuming 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Harpin;fermentation;recombinant E. coli;toxicity determination
期刊名称：ENVIRONMENTAL BIOTECHNOLOGY AND MATERIALS ENGINEERING, PTS 1-3
ISSN： 1022-6680
年卷期： 2011 年 183-185 卷
页码：
收录情况： SCI
摘要： Harpin protein was produced by a recombinant E. coli BL21(DE3)/pET30a(+)hrpNEcc in a 1-ton fermentor, and acute oral toxicity test in rats and guinea pig skin sensitization test for Harpin protein were carried out in order to determine its toxicity. The experimental results show that the protein production is up to 4.78 g/L after 16-18 h of fermentation, and the production cost of this protein is 1.75 yuan/g. Harpin protein produced by the recombinant E. coli strain is non-toxic (LD50 > 5000mg/kg) and has a low allergenicity (rate of sensitization is 0%). The results obtained in this study lay the foundation of the industrial manufacturing and the application on agriculture for Harpin protein.
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