文献类型： 外文期刊
作者： Fang, Ruiqiu 1 ; Chen, Xiaolong 1 ; Shen, Jie 2 ; Wang, Bin 3 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Maize & Featured Upland Crops, Dongyang 322100, Zhejiang, Peoples R China
2.Changzhi Univ, Dept Life Sci, Changzhi 046011, Shanxi, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China
关键词： N6-methyladenosine; Plant m6A editors; Arabidopsis; dLwaCas13a; ALKBH5
期刊名称：PLANT METHODS （ 影响因子：5.1； 五年影响因子：6.1 ）
ISSN：
年卷期： 2023 年 19 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundN6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficult to study the effect of specific RNA methylation.ResultsIn this study, we report the development of Plant m6A Editors (PMEs) using dLwaCas13a (from L. wadei) and human m6A demethylase ALKBH5 catalytic domain. PMEs specifically demethylates m6A of targeted mRNAs (WUS, STM, FT, SPL3 and SPL9) to increase mRNAs stability. In addition, we discovered that a double ribozyme system can significantly improve the efficiency of RNA editing.ConclusionPMEs specifically demethylates m6A of targeted mRNAs to increase mRNAs stability, suggesting that this engineered tool is instrumental for biotechnological applications.
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