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The Effects of Polyadenylation Status on MPFs During In Vitro Porcine Oocyte Maturation

文献类型： 外文期刊

作者： Liu, Huiyu 1 ; Gao, Yan 1 ; Zhai, Bo 2 ; Jiang, Hao 1 ; Ding, Yu 1 ; Zhang, Lianjiang 3 ; Li, Changhong 4 ; Deng, Qiong 1 ;

作者机构： 1.Jilin Univ, Coll Anim Sci, Lab Anim Ctr, 5333 Xian Rd, Changchun 130062, Peoples R China

2.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling, Peoples R China

3.Jilin Agr & Technol Coll, Coll Anim Sci & Technol, Jilin, Jilin, Peoples R China

4.Bai Cheng Normal Univ, Coll Life Sci, Baicheng, Peoples R China

5.Jilin Univ, State & Local Joint Engn Lab Anim Models Human Di, Changchun, Peoples R China

关键词： Porcine oocyte;Polyadenylation;Maturation;Maternal gene;Chromosome alignment

期刊名称：CELLULAR PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.5； 五年影响因子：4.354 ）

ISSN： 1015-8987

年卷期： 2016 年 39 卷 5 期

页码：

收录情况： SCI

摘要： Aims: This study aims to clarify the effects of polyadenylation status on M-phase promoting factors (MPFs) during in vitro porcine oocyte maturation. Methods: In this study, porcine follicular oocytes from large follicles (> 5 millimeter (mm)) and small follicles (< 3 mm) were examined at different follicular developmental stages. The polyadenylation of maternal mRNAs was inhibited by the addition of 3'-deoxyadenosine (3'-da) during the germinal vesicle (GV)(0 h), GV breakdown (GVBD)(18 h), metaphase I (MI)(28 h), and metaphase II (MII) (44 h) stages. In addition, the expression levels and poly-(A) tail lengths of the maternal mRNAs Cyclin 81 and cell division cycle 2 (Cdc2) were determined by real-time quantitative PCR. Immunofluorescence was used to assess spindle formation and chromosome alignment in the examined oocytes. Results: In large-follicle oocytes, the effects of inhibiting polyadenylation caused the percentage of mature to be significantly lower for the treated group than for the untreated group (p < 0.01). 3'-da can significantly improve the rate of small oocyte maturation in vitro and inhibits Cdc2 polyadenylation. Cyclin 81 plays a significant role in promoting the maturation of large-follicle oocytes. Polyadenylation contributes to the formation of dominant follicles and facilitates the selection of dominant follicles. However, the inhibition of adenylation affected spindle formation-related propulsion and chromosome alignment in both large- and small-follicle oocytes. The first polar body could not be extruded in certain large follicles. Conclusions: 3'-da can significantly improve the rate of small oocyte maturation in vitro, but it can also affect spindle formation-related propulsion and chromosome alignment. (C) Copyright 2016 S. Karger AG, Basel

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