Melatonin Protects the Apoptosis of Sheep Granulosa Cells by Suppressing Oxidative Stress via MAP3K8 and FOS Pathway
文献类型： 外文期刊
作者： Zhai, Bo 1 ; Li, Xu 1 ; Zhao, Zhongli 1 ; Cao, Yang 1 ; Liu, Xinxin 1 ; Liu, Zheng 1 ; Ma, Huihai 1 ; Lu, Wenfa 2 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Sci, Changchun 136100, Peoples R China
2.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
关键词： melatonin; apoptosis; sheep; granulosa cells; oxidative stress
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2023 年 14 卷 5 期
页码：
收录情况： SCI
摘要： Melatonin is not only a highly effective active oxygen scavenger but also an important reproductive hormone. Melatonin has a regulatory effect on animal reproduction, especially on the ovaries. It can affect the proliferation and apoptosis of cells in follicles. However, the mechanisms of the dual antioxidation and anti-apoptosis effects of melatonin on granulosa cells are still not clear, especially in sheep. Therefore, we investigated the mechanisms of the protective effect of melatonin against oxidative damage in granulosa cells. At a concentration of 250 mu mol/L, H2O2 promoted granulosa cell apoptosis; however, 10 ng/mL melatonin effectively alleviated the pro-apoptotic effect of H2O2. Furthermore, through the application of high-throughput sequencing technology, we identified 109 significantly differentially expressed genes (35 upregulated and 74 downregulated genes) involved in the protective effect of melatonin against apoptosis. The expression levels of nine related genes, i.e., ATF3, FIBIN, FOS, HSPA6, MAP3K8, FOSB, PET117, DLX2, and TRIB1, changed significantly. MAP3K8 and FOS gene overexpression impacted the protective effect of melatonin in granulosa cells; the two genes exhibited an upstream and downstream regulatory relationship. Our findings indicated that melatonin alleviated H2O2-induced apoptosis in sheep granulosa cells through the MAP3K8-FOS pathway.
- 相关文献
作者其他论文 更多>>
-
Novel perspectives on autophagy-oxidative stress-inflammation axis in the orchestration of adipogenesis
作者：Hong, Chun;Li, Xinming;Huang, Qiuyan;Li, Baohong;Xin, Haiyun;Hu, Bin;Meng, Fanming;Li, Jianhao;Wang, Sutian;Zhang, Kunli;Huang, Qiuyan;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng;Hu, Chuanhuo;Tao, Chenyu;Cao, Yang;Wang, Hai;Deng, Bo;Wang, Sutian
关键词：adipogenesis; autophagy; inflammation; oxidative stress; immune responses
-
Whole genome sequencing identified genomic diversity and candidated genes associated with economic traits in Northeasern Merino in China
作者：Yi, Wenfeng;Hu, Mingyue;Shi, Lulu;Li, Ting;Bai, Chunyan;Yan, Shouqing;Sun, Fuliang;Ma, Huihai;Zhao, Zhongli
关键词：Northeast Merino; whole-genome sequencing; genetic diversity; population structure; selection signatures
-
Effect of HDAC9 on the differentiation of chicken embryonic stem cells into male germ cells
作者：Yu, Yongsheng;Zhang, Qi;Luo, Xiaotong;Zhao, Zhongli;Yu, Li
关键词：cESCs; HDAC9; male germ cells; gene regulation; TMP195.
-
Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress
作者：Chen, Xiaoxin;Liu, Jianqiang;Liu, Yi;Li, Xu;An, Dingjie;Liu, Xiaohui;Zhang, Lichun
关键词：alpha-lipoic acid; antioxidant capacity; semen freezing; rooster sperm ultramicrostructure
-
The comparison of meat yield, quality, and flavor between small-tailed Han sheep and two crossbred sheep and the verification of related candidate genes
作者：Xiao, Cheng;Liu, Yu;Zhao, Wenjun;Liang, Yingjia;Cui, Chao;Yang, Shaoying;Fang, WenWen;Miao, Lisheng;Yuan, Zhiyu;Zhai, Bo;Zhao, Zhongli;Zhang, Lichun;Ma, Huihai;Jin, Haiguo;Cao, Yang;Xiao, Cheng;Xiao, Cheng;Zhao, Wenjun;Lin, Zihan
关键词：small-tailed Han sheep; crossbred sheep; meat yield; quality; volatile compound; PDK4 gene; TMEM273 gene
-
Whole genome sequencing of Aoluguya reindeer (Rangifer tarandus) in China
作者：Shi, Lulu;Shi, Zheng;Hu, Mingyue;Wu, Mao;Sun, Hao;Yan, Shouqing;Quan, Xinjiao;Tian, Laiming;Qin, Lihong;Zhao, Zhongli
关键词：Aoluguya reindeer; whole genome sequencing; SNP; genetic relationship; genetic resource
-
MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle
作者：Wei, Minghong;Xiao, Cheng;Wu, Jian;Qin, Lihong;Liu, Hongliang;Cao, Yang;Zhao, Yumin;Xiao, Cheng;Wu, Jian;Qin, Lihong;Liu, Hongliang;Zhao, Yumin
关键词：Chinese red steppe cattle; MED4; PPAR gamma; Predipocyte differentiation; Lipid metabolism; Meat quality trait
