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Silencing of ADIPOQ Efficiently Suppresses Preadipocyte Differentiation in Porcine

文献类型： 外文期刊

作者： Gao, Yan 1 ; Li, Fujuan 1 ; Zhang, Yonghong 1 ; Dai, Lisheng 1 ; Jiang, Hao 1 ; Liu, Huiyu 1 ; Zhang, Shumin 2 ; Chen, Ch 1 ;

作者机构： 1.Jilin Univ, Coll Anim Sci, Changchun 130062, Peoples R China

2.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling 136100, Peoples R China

关键词： Pig;ADIPOQ;Preadipocytes;Adipocyte differentiation;RNAi

期刊名称：CELLULAR PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.5； 五年影响因子：4.354 ）

ISSN： 1015-8987

年卷期： 2013 年 31 卷 2-3 期

页码：

收录情况： SCI

摘要： Aims: Our study aims to characterize the functions of the ADIPOQ gene in the process of fat deposition of pigs, thereby providing a basis for the use of this gene as a molecular marker for pork quality. Methods: We used healthy Junmu1 piglets less than 7 days of age to establish an in vitro culture system for porcine preadipocytes. Chemically synthesized short hairpin RNAs (shRNA) were transfected into porcine preadipocytes to silence the expression of the ADIPOQ gene. We monitored preadipocyte differentiation and determined the levels of the adipocyte differentiation transcription factors lipoprotein lipase (LPL), peroxisome proliferator-activated receptor. (PPAR.) and adipocyte fatty acid binding protein (AP2) mRNAs to investigate the effects of ADIPOQ on the differentiation of porcine preadipocytes. Results: After transfection, the mRNA and protein levels of the ADIPOQ gene were significantly decreased (P < 0.01), the number of lipid droplets in the adipocytes was significantly reduced, the OD values reflecting the fat content were significantly decreased (P < 0.01), and the levels of LPL, PPAR. and AP2 were significantly reduced (P < 0.01). Conclusions: These results suggest that interference with ADIPOQ gene expression can inhibit the differentiation of porcine preadipocytes. Copyright (C) 2013 S. Karger AG, Basel

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