您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

DNA methylation polymorphism in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max L. Merr.)

文献类型： 外文期刊

作者： Zhong, Xiaofang 1 ; Wang, Yumin 2 ; Liu, Xiaodong 2 ; Gong, Lei 1 ; Ma, Yan 1 ; Qi, Bao 1 ; Dong, Yingshan 2 ; Liu, Bao 1 ;

作者机构： 1.NE Normal Univ, MOE, Key Lab Mol Epigenet, Changchun 130024, Peoples R China

2.Jilin Acad Agr Sci, Changchun 130124, Peoples R China

关键词： Annual wild soybean (Glycine soja);cultivated soybean (G. max);DNA methylation polymorphism;epigenetic variation;evolution;domestication

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2009 年 89 卷 5 期

页码：

收录情况： SCI

摘要： Zhong, X., Wang, Y., Liu, X., Gong, L., Ma, Y., Qi, B., Dong, Y. and Liu, B. 2009. DNA methylation polymorphism in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max L. Merr.). Can. J. Plant Sci. 89: 851-863. To study DNA methylation polymorphism in soybean, 20 and 27 lines of annual wild and cultivated soybeans, respectively, were selected from previously established "Soybean Core Collections in China", and subjected to methylation-sensitive amplified polymorphism (MSAP) analysis. Twenty-seven primer pairs generated 984 CG/CNG methylation sites across the 47 lines, and the data were dissected into methylation-sensitive (MS) and methylation-insensitive(MIS) polymorphisms. The calculated MSP vs. MISP for wild soybeans were 34.65 vs. 34.76%, while corresponding results for the cultivated soybeans were 47.05 vs. 47.15%, indicating higher levels of MSP and MISP in cultivated than in wild soybeans. These results were incongruent with the amplified fragment length polymorphism (AFLP) analysis of the same soybean lines, and suggested enhanced DNA methylation polymorphism due to human selection. All three markers, MSP, MISP and AFLP, enabled clustering of the soybean lines into two distinct groups each predominantly containing wild or cultivated ones. Homology search indicated that 12 out of 24 sequenced MSPs had significant similarities to known-function or predicated genes, suggesting possible functional relevance of the methylation polymorphism. No significant association between MSP and MISP or MSP and AFLP was detected. Our results suggest that DNA methylation polymorphism in soybean has been under both natural and human selections, implicating possible roles of this epigenetic modification in genome evolution and domestication.

  • 相关文献

[1]Progress in chloroplast genome analysis. Liu, Clarke Jihong,Xing Shao-Chen. 2008

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台