您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Viral Suppressor P1/HC-Pro Increases the GFP Gene Expression in Agrobacterium-mediated Transient Assay

文献类型： 外文期刊

作者： Ma, Pengda 1 ; Liu, Jinying 1 ; He, Hongxia 1 ; Yang, Meiying 1 ; Li, Meina 1 ; Zhu, Xiaojuan 1 ; Wang, Xingzhi 1 ;

作者机构： 1.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Peoples R China

2.Jilin Acad Agr Sci, Biotechnol Res Ctr, Changchun 130033, Peoples R China

3.Jilin Agr Univ, Sch Life Sci, Changchun 130018, Peoples R China

关键词： Post-transcriptional gene silencing (PTGS);Suppressor;P1/HC-Pro;Agrobacterium-mediated transient expression system;GFP;Semi-quantitative reverse transcription-PCR

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2009 年 158 卷 2 期

页码：

收录情况： SCI

摘要： More than 20 post-transcriptional gene silencing (PTGS) suppressors have been found since HC-Pro, the first gene silencing suppressor, was found in 1998. The silencing suppressor strongly suggested that gene silencing functions as natural defense mechanisms against viruses. It also represented a valuable tool for the dissection of the gene silencing pathway. We have used P1/HC-Pro RNA silencing suppressor activity to increase green fluorescent protein (GFP) expression in tobacco using an Agrobacterium-mediated transient expression system. P1/HC-Pro stimulated GFP-gene expression but not dsGFP-gene expression was shown by RT-PCR, Northern and Western blot analysis. Expression of the gene silencing suppressor and the target gene provided a new strategy of heterogeneous gene expressing in plants. It may be of commercial significance to produce foreign proteins using plant bioreactors.

  • 相关文献

[1]枯草芽胞杆菌GB519在水稻植株中的定殖及对稻瘟病田间防效. 祁山颜,朱峰,王继春,田成丽,王东元,欧玉苹,刘晓梅,李莉,姜兆远. 2023

[2]小麦miRNA靶基因的体外实验验证. 房春豪,陈志浩,王开,汪晓璐,徐文竞,祁广,马朋涛,韩冉,刘成. 2024

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台