A Viral Suppressor P1/HC-Pro Increases the GFP Gene Expression in Agrobacterium-mediated Transient Assay
文献类型： 外文期刊
作者： Ma, Pengda 1 ; Liu, Jinying 1 ; He, Hongxia 1 ; Yang, Meiying 1 ; Li, Meina 1 ; Zhu, Xiaojuan 1 ; Wang, Xingzhi 1 ;
作者机构： 1.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Peoples R China
2.Jilin Acad Agr Sci, Biotechnol Res Ctr, Changchun 130033, Peoples R China
3.Jilin Agr Univ, Sch Life Sci, Changchun 130018, Peoples R China
关键词： Post-transcriptional gene silencing (PTGS);Suppressor;P1/HC-Pro;Agrobacterium-mediated transient expression system;GFP;Semi-quantitative reverse transcription-PCR
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）
ISSN： 0273-2289
年卷期： 2009 年 158 卷 2 期
页码：
收录情况： SCI
摘要： More than 20 post-transcriptional gene silencing (PTGS) suppressors have been found since HC-Pro, the first gene silencing suppressor, was found in 1998. The silencing suppressor strongly suggested that gene silencing functions as natural defense mechanisms against viruses. It also represented a valuable tool for the dissection of the gene silencing pathway. We have used P1/HC-Pro RNA silencing suppressor activity to increase green fluorescent protein (GFP) expression in tobacco using an Agrobacterium-mediated transient expression system. P1/HC-Pro stimulated GFP-gene expression but not dsGFP-gene expression was shown by RT-PCR, Northern and Western blot analysis. Expression of the gene silencing suppressor and the target gene provided a new strategy of heterogeneous gene expressing in plants. It may be of commercial significance to produce foreign proteins using plant bioreactors.
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