您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Limited tissue culture-induced mutations and linked epigenetic modifications in F-1 hybrids of sorghum pure lines are accompanied by increased transcription of DNA methyltransferases and 5-methylcytosine glycosylases

文献类型： 外文期刊

作者： Zhang, Meishan 2 ; Xu, Chunming 2 ; Yan, Hongyan 4 ; Zhao, Na 2 ; von Wettstein, Diter 1 ; Liu, Bao 2 ;

作者机构： 1.Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA

2.NE Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China

3.Jilin Agr Univ, Dept Agron, Changchun 130118, Peoples R China

4.Jilin Acad Agr Sci, Inst Cereal Crops, Changchun 130124, Peoples R China

关键词： tissue culture;genetic and epigenetic instability;hybrid versus pure line;DNA methyltransferase;5-methylcytosine glycosylases;sorghum

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2009 年 57 卷 4 期

页码：

收录情况： SCI

摘要： In plant tissue culture, developmental disturbance and mutagenic factors are involved in channeling an individual totipotent cell to an intact plant. Comparing a pair of sorghum reciprocal F-1 hybrids with their parental pure lines revealed a dramatic difference in the occurrence of both genetic and DNA methylation alterations in the respective regenerated plants. In contrast to those of the pure lines, regenerated plants of hybrids exhibit significantly enhanced genetic and epigenetic stability. The genetic changes detected by amplified fragment length polymorphism and the DNA methylation alterations detected by methylation-sensitive amplified polymorphism are intimately correlated with each other, suggesting a common mechanism underlying both kinds of instabilities. Markedly altered transcription of genes encoding four putative sorghum DNA methyltransferases and two 5-methylcytosine glycosylases with nucleotide sequences orthologous to Arabidopsis counterparts was induced by tissue culture. The steady-state transcript levels of these genes were negatively correlated with genetic and methylation alterations. A salient observation is that tissue culture-induced transcription of genes encoding DNA methyltransferases and 5-methylcytosine glycosylases in calli and/or regenerated plants of the hybrids was remarkably coordinated, but is largely uncoordinated and stochastically altered in calli and/or regenerated plants of the pure lines. We suggest that the uncoordinated regulation of expression of DNA methyltransferases and 5-methylcytosine glycosylases is a major cause of the high incidence of genetic and DNA methylation alterations in cultures of pure lines, but coordinated up-regulated expression of these enzymes in cultures of the F-1 hybrids fortified their genetic and epigenetic stability.

  • 相关文献

[1]Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice. Lin, Chunjing,Hu, Lanjuan,Yang, Jingjing,Zhou, Tianqi,Xu, Chunming,Liu, Bao,Lin, Chunjing,Lin, Xiuyun,Long, Likun,Xing, Shaochen,Dong, Yingshan,Qi, Bao. 2012

[2]Relative susceptibility of different male-sterile cytoplasms in sorghum to shoot fly, Atherigona soccata. Dhillon, MK,Sharma, HC,Reddy, BVS,Singh, R,Naresh, JS,Kai, Z. 2005

[3]Chinese sorghum genetic resources. Lu, QS,Dahlberg, JA. 2001

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台