Enzymatic synthesis of structured lipids rich in α-linolenic acid: impacts of triacylglycerol profile on physicochemical characteristics
文献类型： 外文期刊
作者： Liu, Xuan 1 ; He, Shi 1 ; Mu, Lixia 2 ; Liu, Jun 2 ; Zhou, Pengfei 2 ; Liao, Sentai 2 ; Wang, Weifei 2 ;
作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China
2.Guangdong Acad Agr Sci, Sericultural & Argi Food Res Inst, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Minist Agr & Rural, 133 Yiheng St,Dongguanzhuang Rd, Guangzhou 510610, Peoples R China
关键词： Enzymatic interesterification; medium- and long- chain triacylglycerols; oxidative stability; structured lipids; triacylglycerols composition; alpha-linolenic acid
期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.1； 五年影响因子：3.5 ）
ISSN： 0950-5423
年卷期： 2024 年 59 卷 10 期
页码：
收录情况： SCI
摘要： Medium- and long-chain triacylglycerols rich in alpha-linolenic acid (ALA-MLCT) are a kind of functional structural lipids that provide omega-3 fatty acid and faster energy supply to human body. To improve the functional and nutritional properties of vegetable oils, we proposed an effective strategy for the synthesis of ALA-MLCT by enzymatic interesterification of coconut oil and flaxseed oil. Lipase UM1 exhibited the highest interesterification activity compared with commercial Lipozyme RM IM, Lipozyme TL IM and Novozym 435. MLCT (76.59%) and ALA-MLCT (45.03%) were obtained under the optimum reaction conditions: substrate molar ratio (coconut oil/flaxseed oil) 1:1, temperature 40 degrees C, Lipase UM1 loading 25 U g-1. Furthermore, we identified 14 characteristic triacylglycerols molecules between enzymatic interesterification and physical blend by orthogonal partial least squares-discrimination analysis (OPLS-DA) model. The content of triunsaturated type triacylglycerols in enzymatic interesterification product (11.29%) was lower than that of physical blend (36.25%). Enzymatic interesterification product had lower solid fat content and 2.44 times oxidative stability than physical blend. These findings provide an effective strategy for the synthesis of functional ALA-MLCT and advance the understanding of the relationship between triacylglycerols composition and physicochemical characteristics. This study established an effective strategy for the synthesis of ALA-MLCT by enzymatic interesterification of coconut oil and flaxseed oil and shed some light on understanding the relationship between triacylglycerol composition and physicochemical properties. This study offers new avenues for the functional ALA-MLCT production as a dietary supplement for human health. image
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