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Constructions and immunogenicity evaluations of two porcine epdemic diarrhea virus-like particle vaccines

文献类型： 外文期刊

作者： Xu, Hong 1 ; Yang, Mengdi 1 ; Liu, Shiyu 1 ; Zheng, Fangyuan 9 ; Li, Yupeng 1 ; Li, Yunchuan 1 ; Wang, Chuanhong 1 ; Qian, Jiali 1 ; Zhao, Yongxiang 1 ; Yang, Shanshan 1 ; Sun, Min 1 ; Song, Xu 1 ; Guo, Rongli 1 ; Zhou, Jinzhu 1 ; Fan, Baochao 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Peoples R China

2.Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Peoples R China

3.Hebei Agr Univ, Coll Vet Med, Baoding 071000, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

5.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

6.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Peoples R China

7.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

8.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China

9.Vazyme Biotech Co Ltd, R&D Dept, Nanjing, Peoples R China

关键词： Porcine epidemic diarrhea virus; Vaccine; Virus-like particles; Immunogenicity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 303 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV), a swine enteropathogenic coronavirus, causing acute diarrhea, dehydration, and up to 100 % mortality in neonatal suckling piglets, leading to huge economic losses in the global swine industry. Vaccination remains the most promising and effective way to prevent and control PEDV. In this study, we produced PEDV virus-like particles (VLPs) composed of S, M, and E proteins with a baculovirus expression system and a mammalian expression system. The S, M, and E proteins were effectively expressed and successfully assembled into VLPs. Subsequently, S subunits and commercially inactivated vaccines were selected and compared with two VLPs vaccines for immune efficacy through mouse immunization. The results showed that both VLPs induced higher levels of IgG, IgA, and neutralizing antibody titers, lymphocyte proliferation indexes and T, B cell ratios. Compared with the baculovirus VLPs, the mammalian VLPs exhibited better effects in inducing neutralizing antibodies, lymphocyte proliferations, and IFN-gamma. These data indicated that the PEDV VLPs vaccine constructed using the mammalian expression system has better immune efficacy and has the potential to serve as a novel PEDV vaccine.

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