文献类型： 外文期刊

作者： Wang, R. Y. ¹ ; Wang, H. G. ² ; Liu, X. Y. ¹ ; Lian, S. ¹ ; Chen, L. ² ; Qiao, Z. J. ² ; McInerney, C. E. ³ ; Wang, L. ² ;

作者机构： 1.Shanxi Agr Univ, Coll Agr, Taigu 030801, Shanxi, Peoples R China

2.Shanxi Acad Agr Sci, Inst Crop Germplasms Resources, Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Taiyuan 030031, Shanxi, Peoples R China

3.Rothamsted Res, Computat & Syst Biol Dept

关键词： Common millet (Panicum miliaceum L.);Drought;Transcription

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2017 年 27 卷 4 期

页码：

收录情况： SCI

摘要： As a well-adapted plant to dry soil and short growing seasons, common millet holds great potential as a drought-resistant crop. Here, we performed a comparative RNA-Seq analysis of two common millet genotypes, Huangmizi (tolerant) and Zhenyuandamizi (sensitive) grown under stress conditions (20% Polyethylene Glycol 6000 with an average molecular weight of similar to 5,400-7,000 solution). Leaves of seedling plants were harvested from a control and also after 1 and 3 h stress treatment and pooled for RNA isolation. Using Illumina paired-end sequencing, approximately 46.5 million clean reads were generated. Following a de novo assembly, a total of 42,240 Unigenes were obtained. A total of 2,301 SSRs and 1,447,148 SNPs were also identified and 75% of Unigenes were annotated. A total of 21,556 and 8,304 Unigenes aligned to the GO and COG databases, respectively. The GO classification showed that the Unigenes were distributed into three categories with 74.30% having biological functions, 76.51% having molecular functions and 79.30% having cellular-level functions. By cross-referencing against KEGGs, 5,535 Unigenes were assigned to 63 metabolic pathways. Additionally, 701 DEGs including 187 up-regulated and 514 down-regulated genes were detected. Transcripts identified in this study will accelerate our understanding of the molecular mechanisms of drought tolerance in common millet.