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Molecular identification and expressive characterization of an olfactory co-receptor gene in the Asian honeybee, Apis cerana cerana

文献类型： 外文期刊

作者： Zhao, Huiting 1 ; Gao, Pengfei 1 ; Zhang, Chunxiang 1 ; Ma, Weihua 1 ; Jiang, Yusuo 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Anim Sci & Technol, Taigu 030801, Shanxi, Peoples R China

2.Shanxi Acad Agr Sci, Inst Hort, Taiyuan 030031, Shanxi, Peoples R China

关键词： gene expression;location analysis;molecular cloning;olfactory receptor

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2013 年 13 卷

页码：

收录情况： SCI

摘要： Olfaction recognition process is extraordinarily complex in insects, and the olfactory receptors play an important function in the process. In this paper, a highly conserved olfactory co-receptor gene, AcerOr2 (ortholog to the Drosophila melanogaster Or83b), cloned from the antennae of the Asian honeybee, Apis cerana cerana Fabricius (Hymenoptera: Apidae), using reverse transcriptase PCR and rapid amplification of cDNA ends. The full-length sequence of the gene was 1763 bp long, and the cDNA open reading frame encoded 478 amino acid residues, including 7 putative transmembrane domains. Alignment analysis revealed that AcerOr2 shares high homology (> 74%) with similar olfactory receptors found in other Hymenoptera species. The amino acid identity with the closely related species Apis mellifera reached 99.8%. The developmental expression analysis using quantitative real-time reverse transcriptase PCR suggested that the AcerOr2 transcript was expressed at a relatively low level in the larval stage, whereas it was expressed broadly in the pupal and adult stages, with a significantly high level on the days just before and after eclosion. In situ hybridization showed that AcerOr2 mRNA was expressed in sensilla placodea and on the basal region of the worker antennal cuticle, in accordance with the previous conclusions that the conserved genes are expressed in most olfactory receptor neurons.

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