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Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain

文献类型： 外文期刊

作者： Wang, Xi 1 ; Li, Hongxia 3 ; Hamid, Mohammod Abdul 1 ; Zhao, Xingbo 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Agribiotechnol, Beijing 100193, Peoples R China

2.Shanxi Acad Agr Sci, Inst Anim Sci & Vet Med, Taiyuan 030000, Peoples R China

3.Shanxi Populat & Family Planning Sci Res Inst, Taiyuan 030006, Peoples R China

关键词： Chicken;FOXP2;splicing variant;immunohistochemistry

期刊名称：ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.869； ）

ISSN： 1683-9919

年卷期： 2012 年 7 卷 8 期

页码：

收录情况： SCI

摘要： The whole cDNA sequence of chicken FOXP2 gene was obtained by 3' and 5' RACE and the length was 3839 bp, getting four splicing variants for protein isoforms (707 AA, 706AA, 687AA, 684AA). The four splicing variants were common to Zang chicken, White Plymouth Rock, Beijing fat chicken, White leghorns and Rhode Island Red by the detection of a combination of PCR and sequencing and according to two main splicing variants, two antibodies were produced to be used to detect equivalent expression in chicken brain such as cortex, striatum, mesencephalon, thalamus and hypothalamus, in White leghorns, (male and female) by immunohistochemistry. The results showed that different FOXP2 mRNA and protein expression had no spatial difference.

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