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Fine mapping of a novel QTL DM9.1 conferring downy mildew resistance in melon

文献类型： 外文期刊

作者： Zhang, Xuejun 1 ; Ling, Yueming 2 ; Yang, Wenli 2 ; Wei, Minghua 4 ; Wang, Zhenzhu 4 ; Li, Meihua 2 ; Yang, Yong 2 ; Liu, Bin 2 ; Yi, Hongping 2 ; Guo, Yang-Dong 1 ; Kong, Qiusheng 2 ;

作者机构： 1.China Agr Univ, Coll Hort, Beijing, Peoples R China

2.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Urumqi, Peoples R China

3.Xinjiang Acad Agr Sci, Hainan Sanya Expt Ctr Crop Breeding, Sanya, Peoples R China

4.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan, Peoples R China

关键词： QTL-seq; QTL; melon; linkage map; downy mildew

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Downy mildew (DM) is a major foliar disease globally causing great economic loss in melon production. Utilizing disease-resistant cultivars is the most efficient approach for disease control, while discovery of disease-resistant genes is crucial for the success of DM-resistant breeding. To address this problem, two F-2 populations were constructed using the DM-resistant accession PI 442177 in this study, and QTLs conferring DM resistance were mapped using linkage map and QTL-seq analysis, respectively. A high-density genetic map with the length of 1096.7 cM and density of 0.7 cM was generated by using the genotyping-by-sequencing data of a F-2 population. A major QTL DM9.1 with the phenotypic variance explained proportion of 24.3-37.7% was consistently detected at the early, middle, and late growth stages using the genetic map. QTL-seq analyses on the two F-2 populations also validated the presence of DM9.1. Kompetitive Allele-Specific PCR (KASP) assay was further carried out to fine map DM9.1 into 1.0 Mb interval. A KASP marker co-segregating with DM9.1 was successfully developed. These results not only provided valuable information for DM-resistant gene cloning, but also offered useful markers for melon DM-resistant breeding programs.

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