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MicroRNA-34c regulates porcine granulosa cell function by targeting forkhead box O3a

文献类型： 外文期刊

作者： Xu Yuan 1 ; Zhang Ai-ling 2 ; Zhang Zhe 1 ; Yuan Xiao-long 1 ; Chen Zan-mou 1 ; Zhang Hao 1 ; Li Jia-qi 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

2.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510642, Guangdong, Peoples R China

关键词： porcine;microRNA-34c;FoxO3a;granulosa cell function

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 9 期

页码：

收录情况： SCI

摘要： Granulosa cells (GCs) are somatic cells of ovary, the behaviors of GCs are important for ovarian function. MicroRNAs (miRNAs) are a class of endogenous 18-24 nucleotide (nt) non-coding RNAs, some of which have been shown to be important regulators of GCs function. miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types. However, the roles of miR-34c in GCs function remain unknown. In this study, we used Annexin V-FITC and EdU assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs. Dual-luciferase reporter assays, quantitative real-time PCR (qRT-PCR) and Western blotting identified Forkhead box O3a (FoxO3a) as a direct target gene of miR-34c. The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs. In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a.

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