您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

p53 and NF kappa B regulate microRNA-34c expression in porcine ovarian granulosa cells

文献类型： 外文期刊

作者： Xu Yuan 1 ; Zhang Ai-ling 2 ; Xiao Guang 1 ; Zhang Zhe 1 ; Chen Zan-mou 1 ; Zhang Hao 1 ; Li Jia-qi 1 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

2.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Guangdong, Peoples R China

关键词： porcine granulosa cells;microRNA-34c expression;promoter;p53;NF kappa B

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 8 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are endogenous 18-24 nucleotide (nt) non-coding RNAs, some of which have been indicated to play key roles in granulosa cells (GCs) function. However, little is known about how the miRNA gene expression itself is regulated in the GCs. Our previous study showed that miR-34c, identified to be a pro-apoptotic and anti-proliferative factor in many cell types, exerted the same effects in porcine GCs. Here, the transcriptional regulation of miR-34c expression in GCs was further investigated. 5' rapid amplification of cDNA ends (RACE) assay indicated that the pri-miR-34c transcription start site was located in 1 556 bp upstream of pre-miR-34c. With dual-luciferase reporter assay, we confirmed a 69 bp core promoter region (-1 799 bp/-1 730 bp) was indispensable for the transcription of miR-34c. Chromatin immunoprecipitation (ChIP) assay demonstrated that p53, p50, and p65 could bind to the transcription factor binding sites within the 69 bp core promoter region. In addition, deletion of transcripition factor binding sites resulted in obvious change of the miR-34c promoter activity. Finally, using overexpression and knockdown of p53, p50, and p65 strategies, we showed that p53 and p50 could positively regulated miR-34c expression, whereas p65 neletively regulated miR-34c expression in GCs. Our results provide new data about the transcription regulatory mechanism of miRNA genes in GCs.

  • 相关文献

[1]Influenza A virus induces p53 accumulation in a biphasic pattern. Shen, Yang,Wang, Xiaodu,Guo, Lin,Qiu, Yafeng,Li, Xiangdong,Yu, Hai,Tong, Guangzhi,Ma, Zhiyong,Xiang, Hua. 2009

[2]Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5 ' Regulatory Region in Chinese Indigenous Cattle Populations. Zhang Ai-ling,Zhang Li,Zhang Liang-zhi,Lan Xian-yong,Zhang Cun-fang,Chen Hong,Zhang Ai-ling,Zhang Li,Zhang Cun-lei,Chen Hong. 2011

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台