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Identification of miRNAs involved in fruit ripening in Cavendish bananas by deep sequencing

文献类型： 外文期刊

作者： Bi, Fangcheng 1 ; Meng, Xiangchun 1 ; Ma, Chao 4 ; Yi, Ganjun 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China

2.Minist Agr, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Guangdong, Peoples R China

3.Guangdong Prov Key Lab Trop & Subtrop Fruit Tree, Guangzhou 510640, Guangdong, Peoples R China

4.Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Prod, IL-50250 Bet Dagan, Israel

关键词： Musa acuminate;Fruit ripening;MiRNA;High-throughput sequencing

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2015 年 16 卷

页码：

收录情况： SCI

摘要： Background: MicroRNAs (miRNAs) are a family of non-coding small RNAs that play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are closely related to the ripening process in model plants. However, the miRNAs that are closely involved in the banana fruit ripening process remain unknown. Methods: Here, we investigated the miRNA populations from banana fruits in response to ethylene or 1-MCP treatment using a deep sequencing approach and bioinformatics analysis combined with quantitative RT-PCR validation. Results: A total of 125 known miRNAs and 26 novel miRNAs were identified from three libraries. MiRNA profiling of bananas in response to ethylene treatment compared with 1-MCP treatment showed differential expression of 82 miRNAs. Furthermore, the differentially expressed miRNAs were predicted to target a total of 815 target genes. Interestingly, some targets were annotated as transcription factors and other functional proteins closely involved in the development and the ripening process in other plant species. Analysis by qRT-PCR validated the contrasting expression patterns between several miRNAs and their target genes. Conclusions: The miRNAome of the banana fruit in response to ethylene or 1-MCP treatment were identified by high-throughput sequencing. A total of 82 differentially expressed miRNAs were found to be closely associated with the ripening process. The miRNA target genes encode transcription factors and other functional proteins, including SPL, APETALA2, EIN3, E3 ubiquitin ligase, beta-galactosidase, and beta-glucosidase. These findings provide valuable information for further functional research of the miRNAs involved in banana fruit ripening.

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