文献类型： 外文期刊
作者： Li, Y. 1 ; Wang, L. 1 ; Zhang, L. Y. 1 ; Liu, Y. L. 1 ; Jiang, Z. Y. 1 ; Cai, R. J. 1 ; Song, C. X. 2 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Guangdong, Peoples R China
2.Guandong Acad Agr Sci, Guangdong Prov Key Lab Livestock Dis Prevent, Guangdong Open Lab Vet Publ Hlth, Inst Anim Hlth, Guangzhou, Guangdong, Peoples R China
关键词： Staphylococcus hyicus;Cytokine;Antibody array;Mice;Disease marker
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Staphylococcal infection is a severe bacterial infection. Finding satisfactory predictable biological markers is essential for the treatment of this condition. In this study, we applied a 32-marker sandwich ELISA-based antibody array to evaluate cytokine changes in Staphylococcus hyicus-inoculated BALB/c mice at 24 and 48 h post infection. Among the cytokines detected, the expression levels of granulocyte colony stimulating factor (G-CSF), interleukin 6 (IL-6), macrophage inflammatory protein 2, and keratinocyte chemoattractant (KC) were increased to levels more than twice higher than those in the control group 24 h after infection, while the expression of interleukin 12p40p70 (IL-12p40p70) was decreased to less than half the level measured in the controls. The expression of G-CSF, IL-6, monocyte chemoattractant protei-5, chemokine ligand 11, and KC was upregulated 48 h post infection, whereas IL-12p40p70 expression was still significantly lower (P < 0.05). Among the detected cytokines, the expression levels of G-CSF, IL-6, and KC were constantly upregulated, while IL-12p40p70 was downregulated. This result was then validated by an ELISA assay analysis, which confirmed that G-CSF, IL-6, KC, and IL-12p40p70 expression levels were specifically modulated after an S. hyicus bacterial infection, while granulocyte monocyte colony stimulation factor, IL-12, and IFN gamma levels were significantly increased after a viral infection. Our study indicated the potential of cytokines G-CSF, IL-6, KC, and IL12p40p70 as markers for detecting S. hyicus infection. The results of this study may provide useful data for the appropriate use of medication following S. hyicus infection.
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