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The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response

文献类型： 外文期刊

作者： Li, Sufen 1 ; Tang, Xuechao 1 ; Zhou, Jinzhu 1 ; Bian, Xianyu 1 ; Wang, Jianxin 1 ; Gu, Laqiang 1 ; Zhu, Xuejiao 1 ; Tao, Ran 1 ; Sun, Min 1 ; Zhang, Xuehan 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Inst Vet Med,Key Lab Vet Biol Engn & Technol,Minis, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225000, Peoples R China

4.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 210014, Peoples R China

5.Jiangsu Acad Agr Sci, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

关键词： Rotavirus; Porcine; Mucosal immunity; NSP4; VP4

期刊名称：VIROLOGY （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0042-6822

年卷期： 2024 年 597 卷

页码：

收录情况： SCI

摘要： Porcine rotavirus (PoRV) is one of the main pathogens causing diarrhea in piglets, and multiple genotypes coexist. However, an effective vaccine is currently lacking. Here, the potential adjuvant of nonstructural protein 4 (NSP4) and highly immunogenic structural protein VP4 prompted us to construct recombinant NSP4 86-175aa (NSP4*) and VP4 26-476aa (VP4*) proteins, combine them as immunogens to evaluate their efficacy. Results indicated that NSP4* enhanced systemic and local mucosal responses induced by VP4*. The VP4*-IgG, VP4*-IgA in feces and IgA-secreting cells in intestines induced by the co -immunization were significantly higher than those induced by VP4* alone. Co -immunization of NSP4* and VP4* also induced strong cellular immunity with significantly increased IFN- lambda than the single VP4*. Summarily, the NSP4* as a synergistical antigen exerted limited effects on the PoRV NAbs elevation, but conferred strong VP4*-specific mucosal and cellular efficacy, which lays the foundation for the development of a more effective porcine rotavirus subunit vaccine.

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