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Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae

文献类型： 外文期刊

作者： Zhao, Xiangjie 1 ; Li, Lin 1 ; Kuang, Zheshi 2 ; Luo, Guoqing 2 ; Li, Bing 1 ;

作者机构： 1.S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510610, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou, Guangdong, Peoples R China

3.Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian, Jiangsu, Peoples R China

关键词： silkworm (Bombyx mori L.) pupa;allergen;chitinase;paramyosin;western blot;MALDI-TOF-MS

期刊名称：Central European Journal of Immunology （ 影响因子：2.085； 五年影响因子：2.286 ）

ISSN： 1426-3912

年卷期： 2015 年 40 卷 1 期

页码：

收录情况： SCI

摘要： This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin E (IgE)-binding proteins by western blotting, and allergens specific for silkworm pupa consumption-caused allergy were visualised with the ECL reagents. The selected allergen proteins were further identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding reaction. Analysis of the sequence homology of the two proteins using the Allergen Online database indicated that chitinase and paramyosin shared 24.8% and 62.8% sequence homology with known allergens Der f 18 (Dennatophagoides farinae) and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed light on the understanding and treatment of silkworm pupa allergy.

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