Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae
文献类型: 外文期刊
作者: Zhao, Xiangjie 1 ; Li, Lin 1 ; Kuang, Zheshi 2 ; Luo, Guoqing 2 ; Li, Bing 1 ;
作者机构: 1.S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510610, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou, Guangdong, Peoples R China
3.Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian, Jiangsu, Peoples R China
关键词: silkworm (Bombyx mori L.) pupa;allergen;chitinase;paramyosin;western blot;MALDI-TOF-MS
期刊名称:Central European Journal of Immunology ( 影响因子:2.085; 五年影响因子:2.286 )
ISSN: 1426-3912
年卷期: 2015 年 40 卷 1 期
页码:
收录情况: SCI
摘要: This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin E (IgE)-binding proteins by western blotting, and allergens specific for silkworm pupa consumption-caused allergy were visualised with the ECL reagents. The selected allergen proteins were further identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding reaction. Analysis of the sequence homology of the two proteins using the Allergen Online database indicated that chitinase and paramyosin shared 24.8% and 62.8% sequence homology with known allergens Der f 18 (Dennatophagoides farinae) and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed light on the understanding and treatment of silkworm pupa allergy.
- 相关文献
作者其他论文 更多>>
-
Exploration of the immune response of grass carp (Ctenopharyngodon idellus) erythrocytes during bacterial infection
作者:Yang, Shiyi;Bai, Yanhan;Tao, Junjie;Tu, Chengming;Huang, Xiaoman;Zhang, Linpeng;Liu, Lihan;Li, Lin;Qin, Zhendong;Chen, Bing
关键词:GcRBCs; Inflammation; ROS; Antioxidant system; Apoptosis
-
Migration, Health Risks, and Source Apportionment of Heavy Metals in Agricultural Soil-Dustfall-Crop Systems in a Typical Watershed of the Taihu Region, China
作者:Chen, Lian;Tang, Cuiming;Luo, Guoqing;Wang, Zhenjiang;Lin, Sen;Zhong, Jianwu;Li, Zhiyi;Wang, Yuan;Zhou, Shenglu;Tang, Cuiming;Luo, Guoqing;Wang, Zhenjiang
关键词:Heavy metal; Soil-dustfall-crop system; Health risks; Source apportionment; Quantitative relationships
-
Effects of Dietary Supplementation with Mulberry Leaf Powder on the Growth Performance, Lipid Metabolism Parameters, Immunity Indicators, and Gut Microbiota of Dogs
作者:Yu, Aiying;Tang, Cuiming;Wang, Yuan;Chen, Lian;Li, Zhiyi;Luo, Guoqing;Zhong, Jianwu;Wang, Zhenjiang;Lin, Sen;Yu, Aiying;Fang, Zhengfeng;Wang, Sutian
关键词:dogs; mulberry leaf powder; lipid metabolism; gut microbiota; immunity
-
Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea
作者:Dai, Fanwei;Luo, Guoqing;Wang, Zhenjiang;Xu, Yujuan;Wang, Dan;Zhong, Jianwu;Lin, Sen;Chen, Lian;Li, Zhiyi;Wang, Yuan;Tang, Cuiming;Dai, Fanwei;Luo, Guoqing;Wang, Zhenjiang;Tang, Cuiming;Zhuo, Xiaokang;Zhuo, Xiaokang;Zheng, Tangchun;Zhang, Diyang;Li, Yuanyuan;Zheng, Qinyao;Liu, Zhong-Jian;Wang, Li;Wang, Li;Zhang, Zhiyong
关键词:domestication; evolutionary history; flowering time; genome; mulberry
-
Catechol derivatives interact with bovine serum albumin: Correlation of non-covalent interactions and antioxidant activity
作者:Weng, Longmei;Li, Lin;Ji, Lili;Wu, Ming;Wu, Yi;Zhang, Xia;Li, Bing;Chen, Zhiyi;Li, Lin;Yang, Haitao
关键词:Catechol; 1-monocaffeoyl glycerol; BSA; Multi-spectroscopic analysis; Multifunctional wavefunction; Antioxidant property
-
A novel methodological framework for risk zonation and source-sink response concerning heavy-metal contamination in agroecosystems
作者:Chen, Lian;Tang, Cuiming;Luo, Guoqing;Wang, Zhenjiang;Lin, Sen;Zhong, Jianwu;Li, Zhiyi;Wang, Yuan;Zhou, Shenglu;Tang, Cuiming;Luo, Guoqing;Wang, Zhenjiang;Zhou, Shenglu;Tang, Cuiming
关键词:GIS-DCMA; LISA; Assessment criteria; Risk zonation; Source-sink
-
Effects of Simulated In Vitro Digestion on the Structural Characteristics, Inhibitory Activity on alpha-Glucosidase, and Fermentation Behaviours of a Polysaccharide from Anemarrhena asphodeloides Bunge
作者:Chen, Juncheng;Chen, Juncheng;Lan, Meijuan;Zhang, Xia;Li, Bing;Jiao, Wenjuan;Chen, Zhiyi;Li, Lin
关键词:polysaccharide; Anemarrhena asphodeloides Bunge; salivary-gastrointestinal digestion; gut microbiota