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Rapid Identification of Peanut Hybrids with SSR-PCR

文献类型: 外文期刊

作者: Hong Yanbin 1 ; Liu Hong 3 ; Li Xingyu 1 ; Chen Xiaoping 2 ; Liang Xuanqiang 2 ; Huang Shangzhi 1 ;

作者机构: 1.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Natl Ctr Oilseeds Crop Improvement, South China Peanut Sub Ctr, Crops Res Inst, Guangzhou 510640, Guangdong, Peoples R China

3.South China Agr Univ, Coll Agr, Guangzhou Div, New Plant Variety Test Ctr,Minist Agr, Guangzhou 510642, Guangdong, Peoples R China

关键词: Peanut;SSR;PCR;hybrid;identification

期刊名称:RESEARCH JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.233; 五年影响因子:0.252 )

ISSN: 0973-6263

年卷期: 2015 年 10 卷 1 期

页码:

收录情况: SCI

摘要: To fulfill high efficiency peanut hybrid identification, an SSR-PCR rapid detection system was established by simplifying the process of DNA template preparation. After that, true hybrids from six F-1 populations were identified by morphological observation and SSR-PCR. The result showed that true and pseudo peanut hybrids were difficultly distinguished by morphological observation when hybrid parents were similar in morphology whereas it was easy by SSR-PCR. In the six F1 populations, 38-56% of putative hybrids were identified as true hybrids by SSR-PCR. Hybrid rates of peanut might vary among different combinations even in reciprocal crosses.

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