文献类型： 外文期刊

作者： Xiang Xu ¹ ; Zhang YiShun ² ; Huang ShangZhi ² ; Ou Liangxi ¹ ; Qiu Yanping ¹ ; Li Zhiqiang ¹ ; Chen Jiezhen ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

2.Zhongshan Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

关键词： virtual northern blot;S-adenosylmethionine synthetase gene (SAM gene)

期刊名称：PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON STRUCTURAL AND FUNCTIONAL GENOMICS OF HORTICULTURAL PLANTS

ISSN： 0567-7572

年卷期： 2007 年 763 期

页码：

收录情况： SCI

摘要： To study the molecular mechanisms associated with embryo abortion in seed-shriveled litchi varieties, suppressive subtraction hybridization (SSH) was used to investigate differential gene expression of normal-seeded and seed-shriveled embryos during stages of I to II of fruit-set. The differential expression cDNA library, representing the specific cDNA for aborting embryos of 'GuiWei' litchi, was built using the embryos from different fruit-set stages, with the normal-seeded embryo as the driver and the seed-shriveled embryo as the tester. After testing by Virtual Northern, 3 positive clones were obtained. We isolated 3 cDNAs from the 3 clones, which were 342, 599 and 391 nucleotides long and were designated Lce1, Lce2 and Lce3, respectively. The Lce1 amino acid sequence showed 97% homology with ubiquitin-conjugated protein of Capsicum and Solanum, Lce2 showed 94% homology with a Brassica S-adenosylmethionine synthetase and Lce3 showed 98% homology with a Zea mays methionine adenosyltransferase. These are the first reported sequences in litchi. The full-length sequence was obtained by PCR amplification and a complete cDNA for SAM synthetase gene was obtained. The sequence was 1515 base pairs (bp) with an opening reading frame (ORF) encoding 393 amino acids (GenBank accession NO: AY259227). This SAM synthetase gene in embryos of 'Guiwei' litchi may be associated with abortion.