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Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

文献类型: 外文期刊

作者: Li, D. M. 1 ; Lu, F. B. 1 ; Zhu, G. F. 1 ; Sun, Y. B. 1 ; Liu, H. L. 1 ; Liu, J. W. 1 ; Wang, Z. 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Guangdong Key Lab Ornamental Plant Germplasm Inno, Environm Hort Res Inst, Guangzhou, Guangdong, Peoples R China

关键词: Flowering locus T;Flowering transition;Gene expression;Phalaenopsis orchids;Warm day and cool night conditions

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2014 年 13 卷 3 期

页码:

收录情况: SCI

摘要: Warm day and cool night conditions significantly induce reproductive spike formation in Phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. Flowering locus T (FT) plays important roles in flowering induction in several plants. To explore spike induction by warm days and cool nights in Phalaenopsis orchids, we isolated the FT (PhFT) from Phalaenopsis hybrid Fortune Saltzman. The cDNA of PhFT was 809-bp long and contained a 531-bp open reading frame encoding a putative protein of 176 amino acids, a 58-bp 5'-untranslated region (UTR), and a 220-bp 3'-UTR. The predicted molecular mass of PhFT was 19.80 kDa, with an isoelectric point of 8.68. The PhFT was predicted to possess the conserved functional regions of the phosphatidy lethanolamine-binding protein superfamily. Nucleotide sequence data indicated that PhFT contained 3 introns and 4 exons. Sequence alignment and phylogenetic analyses of PhFT revealed high homology to the FT proteins of Cymbidium goeringii and Oncidium Gower Ramsey. Quantitative real-time polymerase chain reaction analysis indicated that PhFT mRNA was expressed in roots, apical leaves, mature leaves, and flowers. In flowers, PhFT was expressed more in developing floral buds than in mature flowers and was predominantly expressed in ovaries and petals. Ectopic expression of PhFT in Arabidopsis ft-1 mutants showed novel early-flowering phenotypes that lost their siliques. Our results indicated that the ectopic expression of PhFT could partially complement the late flowering defect in transgenic Arabidopsis ft-1 mutants. Our findings suggest that PhFT is a putative FT homolog in Phalaenopsis plants that regulates flowering transition.

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