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Exploring the formation mechanism of short-tailed phenotypes in animals using mutant mice with the TBXT gene c.G334T developed by CRISPR/Cas9

文献类型： 外文期刊

作者： Su, Hong 1 ; Zhi, Dafu 1 ; Song, Yongli 5 ; Yang, Yanyan 4 ; Wang, Daqing 1 ; Li, Xiunan 1 ; Cao, Guifang 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot, Peoples R China

2.Inst Inner Mongolia Autonomous Reg, Anim Embryo & Dev Engn Key Lab Higher Educ, Hohhot, Peoples R China

3.Inner Mongolia Autonomous Reg Key Lab Basic Vet Me, Hohhot, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb, Hohhot, Peoples R China

5.Inner Mongolia Univ, Coll Life Sci, Hohhot, Peoples R China

关键词： TBXT gene; CRISPR/Cas9; Short-tailed phenotype; Wnt signaling pathway

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2024 年 910 卷

页码：

收录情况： SCI

摘要： With the change in diet structure, individuals prefer to consume mutton with less fat. However, sheep tail has a lot of fat. We identified a breed of low-fat short-tailed sheep (i.e., Hulunbuir short-tailed sheep). It is necessary to develop an animal model that can promote research on the potential mechanisms of the short-tail phenotype in sheep, which results from the TBXT gene c.G334T mutation. To create animal models, we selected mice as experimental animals. Mouse embryos lacking the TBXT protein, which crucially regulates mouse embryonic development, cannot develop normally. We utilized CRISPR/Cas9 gene editing technology to generate sitespecific mutation (c.G334T) in the TBXT gene of mice, and found that the mouse TBXT mutation (c.G334T) leads to a short-tail phenotype. Furthermore, we investigated the interaction between TBXT and Wnt signaling pathways. The expressions of TBXT, Axin2, Dkk1, Wnt3, Wnt3a, and Wnt5a were discovered to be significantly different between mutant embryos and wild embryos by obtaining mouse embryos at various developmental stages and examining the expression relationship between the TBXT and Wnt signaling pathway-related components in all of these embryos. Therefore, as a transcription factor, TBXT regulates the expression of the aforementioned Wnt signaling pathway components by forming a regulatory network for the normal development of mouse embryos. This study enriches the research on the functional role of the TBXT in the development of mouse embryos and the mechanism by which the short-tailed phenotype in sheep develops.

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