Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice
文献类型： 外文期刊
作者： Liu, Yan 1 ; Liu, Bin 2 ; Zhu, Xiaoyuan 3 ; Yang, Jianyuan 1 ; Bordeos, Alicia 1 ; Wang, Guoliang 4 ; Leach, Jan E. 5 ; L 1 ;
作者机构： 1.Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, Manila, Philippines
2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou, Guangdong, Peoples R China
4.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA
5.Colorado State Univ, Ft Collins, CO 80537 USA
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期： 2013 年 126 卷 4 期
页码：
收录情况： SCI
摘要： The major quantitative trait locus qBR9.1 confers broad-spectrum resistance to rice blast, and was mapped to a similar to 69.1 kb region on chromosome 9 that was inherited from resistant variety Sanhuangzhan No 2 (SHZ-2). Within this region, only one predicted disease resistance gene with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains was found. Specific markers corresponding to this gene cosegregated with blast resistance in F-2 and F-3 populations derived from crosses of susceptible variety Texianzhan 13 (TXZ-13) to SHZ-2 and the resistant backcross line BC-10. We tentatively designate the gene as Pi56(t). Sequence analysis revealed that Pi56(t) encodes an NBS-LRR protein composed of 743 amino acids. Pi56(t) was highly induced by blast infection in resistant lines SHZ-2 and BC-10. The corresponding allele of Pi56(t) in the susceptible line TXZ-13 encodes a protein with an NBS domain but without LRR domain, and it was not induced by Magnaporthe oryzae infection. Three new cosegregating gene-specific markers, CRG4-1, CRG4-2 and CRG4-3, were developed. In addition, we evaluated polymorphism of the gene-based markers among popular varieties from national breeding programs in Asia and Africa. The presence of the CRG4-2 SHZ-2 allele cosegregated with a blast-resistant phenotype in two BC2F1 families of SHZ-2 crossed to recurrent parents IR64-Sub1 and Swarna-Sub1. CRG4-1 and CRG4-3 showed clear polymorphism among 19 varieties, suggesting that they can be used in marker-assisted breeding to combine Pi56(t) with other target genes in breeding lines.
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