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A Dominant Major Locus in Chromosome 9 of Rice (Oryza sativa L.) Confers Tolerance to 48 degrees C High Temperature at Seedling Stage

文献类型： 外文期刊

作者： Wei, He 1 ; Liu, Jianping 1 ; Wang, Ya 1 ; Huang, Nongrong 2 ; Zhang, Xiaobo 1 ; Wang, Liangchao 1 ; Zhang, Jiwen 1 ; Tu, 1 ;

作者机构： 1.Zhejiang Univ, Inst Crop Sci, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 510640, Peoples R China

关键词： cosegregation;gene mapping;OsHTAS;PCR-RFLP;RBsp1407

期刊名称：JOURNAL OF HEREDITY （ 影响因子：2.645； 五年影响因子：3.069 ）

ISSN： 0022-1503

年卷期： 2013 年 104 卷 2 期

页码：

收录情况： SCI

摘要： In an earlier greenhouse screening, we identified a local indica cultivar HT54 tolerant to high temperature at both seedling and grain-filling stages. In this study, we develop an optimized procedure for fine assessment of this heat tolerance. The results indicated that HT54 seedlings could tolerate high temperature up to 48 C for 79h. The genetic analysis of F-1 and F-2 offspring derived from the cross between HT54 and HT13, a heat-sensitive breeding line, reveals that the heat tolerance of HT54 was controlled by a dominant major locus, which has been designated as OsHTAS (Oryza sativa heat tolerance at seedling stage). This locus was mapped on rice chromosome 9 within an interval of 420kb between markers of InDel5 and RM7364. The determined candidate ZFP gene has been confirmed to be cosegregated with a single nucleotide polymorphism (SNP) developed PCR-restriction fragment length polymorphism (RFLP) marker RBsp1407 in its promoter region. Another heat tolerance-associated SNP was identified in the first intron of its 5-untranslated region. The existence of these SNPs thereby indicated that the OsHTAS locus contains at least two alleles. We named the one from HT54 as OsHTAS(a) and the one from HT13 as OsHTAS(b). Further dynamic expression analysis demonstrated that OsHTAS(a) was actively responsive to 45 C high temperature stress compared with the OsHTAS(b) allele.

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