您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and comparison of microRNAs from skeletal muscle and adipose tissues from two porcine breeds

文献类型： 外文期刊

作者： Li, Hong-Yi 1 ; Xi, Qian-Yun 1 ; Xiong, Yuan-Yan; Liu, Xiao-Long 1 ; Cheng, Xiao 1 ; Shu, Gang 1 ; Wang, Song-Bo 1 ; W 1 ;

作者机构： 1.S China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

2.S China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, People

关键词： adipose tissue;Landrace pig;Lantang pig;porcine miRNAs;skeletal muscle

期刊名称：ANIMAL GENETICS （ 影响因子：3.169； 五年影响因子：3.058 ）

ISSN： 0268-9146

年卷期： 2012 年 43 卷 6 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that negatively regulate gene expression at the post-transcriptional level. Although an increasing number of porcine miRNAs recently have been identified, research has yet to identify the full repertoire of miRNAs in pig skeletal and adipose tissues and their differences between breeds. We extracted small RNA from skeletal muscle and adipose tissues of Landrace and Lantang pigs, and the expression of a total of 184 known porcine miRNAs (113 from Solexa sequencing and 171 from miRNA chip hybridization) as well as 521 novel miRNA candidates was detected. Moreover, 20 miRNAs were selected randomly from the 184 miRNAs and analysed by quantitative real-time PCR to confirm the aforementioned results. In the skeletal muscle tissues, 21 miRNAs were up-regulated in Lantang and another 33 were highly expressed in Landrace pigs. In the adipose tissues, 25 miRNAs were down-regulated in Lantang and another 23 were lowly expressed in Landrace pigs. miRNA divergence between tissues was also detected in this study. Ten miRNAs were highly expressed in the skeletal muscle tissue in comparison with adipose tissue, and another 10 miRNAs exhibited the opposite expression profile. To investigate the regulatory mechanism of the miRNAs in muscle and adipose tissues, the 10 miRNAs with the most divergent expression profiles were functionally categorized using the Kyoto Encyclopedia of Genes and Genomes database. Most of the miRNAs strongly corresponded to myogenesis and adipogenesis processes. In addition, 84 of the 521 miRNA candidates were potentially porcine-specific miRNAs. This study adds new valuable information to comparative miRNA profiles of skeletal muscle and adipose tissues in porcine species. The great diversity of miRNA composition and expression levels both between breeds and between tissues suggests that a complex regulatory network exists in porcine subcutaneous fat development.

  • 相关文献

[1]CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs. Qiu, Yue-qin,Yang, Xue-fen,Ma, Xian-yong,Xiong, Yun-xia,Tian, Zhi-mei,Fan, Qiu-li,Wang, Li,Jiang, Zong-yong. 2017

[2]INFLAMMATION-RELATED OXIDATIVE STRESS IN WHITE ADIPOSE TISSUES OF AN INBRED OBESE PIG. Yang, X. F.,Jiang, Z. Y.,Tian, Z. M.,Qiu, Y. Q.,Wang, L.,Gao, K. G.,Hu, Y. J.,Ma, X. Y.. 2017

[3]Effect of Dietary Energy on Growth Performance and Fat Deposition of Lantang Growing Pigs. Yang, X. F.,Jiang, Z. Y.,Ma, X. Y.,Zheng, C. T.,Lin, Y. C.,Wang, S. K.,Jiang, S. Q.,Chen, F.,Zhou, G. L.,Yang, X. F..

[4]Copy Number Variation in SOX6 Contributes to Chicken Muscle Development. Lin, Shudai,Lin, Xiran,Zhang, Zihao,Jiang, Mingya,Nie, Qinghua,Zhang, Xiquan,Rao, Yousheng. 2018

[5]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[6]Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua. 2017

[7]MicroRNAs Involved in Skeletal Muscle Differentiation. Zhang, Xiquan. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台