Deep Sequencing-Based Transcriptome Analysis of Chicken Spleen in Response to Avian Pathogenic Escherichia coli (APEC) Infection
文献类型： 外文期刊
作者： Nie, Qinghua 1 ; Sandford, Erin E. 2 ; Zhang, Xiquan 1 ; Nolan, Lisa K. 5 ; Lamont, Susan J. 2 ;
作者机构： 1.S China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China
2.Iowa State Univ, Dept Anim Sci, Ames, IA USA
3.Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
4.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China
5.Iowa State Univ, Coll Vet Med, Dept Vet Microbiol, Ames, IA USA
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2012 年 7 卷 7 期
页码：
收录情况： SCI
摘要： Avian pathogenic Escherichia coli (APEC) leads to economic losses in poultry production and is also a threat to human health. The goal of this study was to characterize the chicken spleen transcriptome and to identify candidate genes for response and resistance to APEC infection using Solexa sequencing. We obtained 14422935, 14104324, and 14954692 Solexa read pairs for non-challenged (NC), challenged-mild pathology (MD), and challenged-severe pathology (SV), respectively. A total of 148197 contigs and 98461 unigenes were assembled, of which 134949 contigs and 91890 unigenes match the chicken genome. In total, 12272 annotated unigenes take part in biological processes (11664), cellular components (11927), and molecular functions (11963). Summing three specific contrasts, 13650 significantly differentially expressed unigenes were found in NC Vs. MD (6844), NC Vs. SV (7764), and MD Vs. SV (2320). Some unigenes (e. g. CD148, CD45 and LCK) were involved in crucial pathways, such as the T cell receptor (TCR) signaling pathway and microbial metabolism in diverse environments. This study facilitates understanding of the genetic architecture of the chicken spleen transcriptome, and has identified candidate genes for host response to APEC infection.
- 相关文献
作者其他论文 更多>>
-
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
作者：Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua
关键词：TMEM182; Fat formation; Metabolomics; Lipidomics
-
Using multiomics to explore the weight differences between genders in Muscovy ducks
作者：Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Zhu, Weijian;Ji, Congliang
关键词：Muscovy duck; sexual dimorphism; growth and development; RNA-seq; 16S rRNA sequencing technology
-
PMAIP1 promotes J subgroup avian leukosis virus replication by regulating mitochondrial function
作者：Zhao, Yongxia;Zhao, Changbin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Deng, Yuelin
关键词：ALV-J; PMAIP1; mitochondrial function; immune; DF-1
-
Live chicken body fat measurement technology based on bio-electrical impedance
作者：Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Deng, Yuelin
关键词：BIA; Electrical impedance; Fat content; Broilers
-
Whole-transcriptome sequencing revealed the ceRNA regulatory network during the proliferation and differentiation of goose myoblast
作者：Zhang, Xiquan;Luo, Wen;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen;Xiao, Liangchao;Chen, Jiahui;He, Xueying;Zhang, Xiquan;Luo, Wen
关键词：Shitou goose; skeletal muscle; whole-transcriptome sequencing; lncRNA; ceRNA network
-
LncEDCH1 g.1703613 T>C regulates chicken carcass traits by targeting miR-196-2-3p
作者：Yuan, Rongshuai;Cai, Bolin;Ma, Manting;Zhao, Changbin;Xian, Yuanrong;Nie, Qinghua;Zhang, Xiquan;Zhang, Dexiang;Zhang, Dexiang
关键词：LncEDCH1; miR-196-2-3p; single nucleotide polymorphism; carcass trait; chicken
-
Weighted single-step GWAS identified candidate genes associated with carcass traits in a Chinese yellow-feathered chicken population
作者：Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Pan, Rongyang;Qi, Lin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen
关键词：Chinese yellow-feathered chicken; weighted single-step GWAS; carcass traits; SNP
