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Development and application of a recombinase-aided amplification and lateral flow assay for rapid detection of pseudorabies virus from clinical crude samples

文献类型： 外文期刊

作者： Guo, Zhenhua 1 ; Xing, Guangxu 1 ; Li, Peng 4 ; Jin, Qianyue 1 ; Lu, Qingxia 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou, Peoples R China

2.Peking Univ, Beijing, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

4.Iowa State Univ, Vet Diagnost & Prod Anim Med, Ames, IA USA

5.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

关键词： Pseudorabies virus; Isothermal amplification; Recombinase-aid amplification; Lateral flow assay; Point of care testing

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 224 卷

页码：

收录情况： SCI

摘要： Pseudorabies virus (PRV) is an economically important viral agent affecting the swine industry in China. Ac-curate, rapid and simple detection is critical to PRV control and eradication. In the present study, a visible and low equipment-dependent recombinase-aid amplification assay integrated with lateral flow assay (RAA-LFA) was successfully developed to detect the PRV against the gE gene. The RAA-LFA did not react with the other swine pathogens, indicating the method has a good specificity. The limit of detection (LOD) for this RAA-LFA method was 21 copies per reaction against standard plasmids containing gE gene. Notably, the RAA-LFA can detect as low as 6.0 x 100 50 % tissue culture infective dose (TCID50) viral titer per reaction under nucleic-acid-extraction free condition. Clinical detection showed that the results detected by RAA-LFA were completely consistent with that of the qPCR assay. Taken together, the developed PRV RAA-LFA method provides approachability, com-parable accuracy and sensitivity tool for PRV point-of-care testing (POCT), which is valuable to PRV control in areas where equipment and personnel resources are scarce.

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