文献类型： 外文期刊

作者： Li, Dawei ¹ ; Cuevas, Hugo E. ¹ ; Yang, Luming ¹ ; Li, Yuhong ¹ ; Garcia-Mas, Jordi ⁴ ; Zalapa, Juan ¹ ; Staub, Jack E ¹ ;

作者机构： 1.Univ Wisconsin, Dept Hort, Madison, WI 53706 USA

2.NW A & F Univ, Hort Coll, Yangling 712100, Peoples R China

3.ARS, USDA, Trop Agr Res Stn, Mayaguez, PR 00680 USA

4.IRTA, Ctr Res Agr Genom, CSIC IRTA UAB, Bellaterra 08193, Barcelona, Spain

5.Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit,Hort Dept, Madison, WI 53706 USA

6.Utah State Univ, USDA, ARS, Forage & Range Res Lab, Logan, UT 84322 USA

7.NE Agr Univ, Hort Coll, Harbin 150030, Peoples R China

8.W Virginia State Univ, Dept Biol, Institute, WV 25112 USA

9.Guang

关键词： Cucumber;Melon;Cucumis;Microsatellite;Comparative mapping;Chromosome evolution

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2011 年 12 卷

页码：

收录情况： SCI

摘要： Background: Cucumber, Cucumis sativus L. (2n = 2 x = 14) and melon, C. melo L. (2n = 2 x = 24) are two important vegetable species in the genus Cucumis (family Cucurbitaceae). Both species have an Asian origin that diverged approximately nine million years ago. Cucumber is believed to have evolved from melon through chromosome fusion, but the details of this process are largely unknown. In this study, comparative genetic mapping between cucumber and melon was conducted to examine syntenic relationships of their chromosomes. Results: Using two melon mapping populations, 154 and 127 cucumber SSR markers were added onto previously reported F(2)- and RIL-based genetic maps, respectively. A consensus melon linkage map was developed through map integration, which contained 401 co-dominant markers in 12 linkage groups including 199 markers derived from the cucumber genome. Syntenic relationships between melon and cucumber chromosomes were inferred based on associations between markers on the consensus melon map and cucumber draft genome scaffolds. It was determined that cucumber Chromosome 7 was syntenic to melon Chromosome I. Cucumber Chromosomes 2 and 6 each contained genomic regions that were syntenic with melon chromosomes III+V+XI and III+VIII+XI, respectively. Likewise, cucumber Chromosomes 1, 3, 4, and 5 each was syntenic with genomic regions of two melon chromosomes previously designated as II+XII, IV+VI, VII+VIII, and IX+X, respectively. However, the marker orders in several syntenic blocks on these consensus linkage maps were not co-linear suggesting that more complicated structural changes beyond simple chromosome fusion events have occurred during the evolution of cucumber. Conclusions: Comparative mapping conducted herein supported the hypothesis that cucumber chromosomes may be the result of chromosome fusion from a 24-chromosome progenitor species. Except for a possible inversion, cucumber Chromosome 7 has largely remained intact in the past nine million years since its divergence from melon. Meanwhile, many structural changes may have occurred during the evolution of the remaining six cucumber chromosomes. Further characterization of the genomic nature of Cucumis species closely related to cucumber and melon might provide a better understanding of the evolutionary history leading to modern cucumber.