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Selection of reference genes for studies of porcine endometrial gene expression on gestational day 12

文献类型： 外文期刊

作者： Wang, Shouqi 1 ; Li, Jiaqi 1 ; Zhang, Ailing 1 ; Liu, Manqing 1 ; Zhang, Hao 1 ;

作者机构： 1.S China Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China; S China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

关键词： Reference gene;Real time quantitative RT-PCR;Endometrium;Pig

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2011 年 408 卷 2 期

页码：

收录情况： SCI

摘要： Comparing gene expression patterns in the endometrium on gestational day 12 (GD12) between Erhualian (ER) and Landrace x Large White (LL) pigs is helpful to understand the biological mechanisms of fecundity. Selecting genes that have stable expression levels as the internal standards in a comparative study is essential for identifying real gene-specific variation by quantitative RT-PCR (qRT-PCR). Five genes expressed in sow endometria on GD12 were evaluated for their suitability as internal control for relative quantification by qRT-PCR. These genes were beta-actin (ACTB), beta-2-microglobulin (B2M), phosphoglycerate kinase 1 (PGK1), RNA polymerase II polypeptide G (RPG), and ribosomal protein S20 (RPS20), which represent different functional classes. Our results indicated that ACTB, B2M, and PGK1 were not suitable as internal standards for normalization because of their huge variability between the two breeds. RPS20 and RPG were most stable, and the former is recommended to serve as the internal standard when the use of multiple housekeeping genes is unpractical. (C) 2011 Elsevier Inc. All rights reserved.

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