Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice
文献类型： 外文期刊
作者： Liu, Yan 1 ; Zhu, Xiao Yuan 1 ; Zhang, Shaohong 3 ; Bernardo, Marichu 1 ; Edwards, Jeremy 4 ; Galbraith, David W. 5 ;
作者机构： 1.Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, Manila, Philippines
2.NW A&F Univ, Coll Agron, Yangling, Shaanxi, Peoples R China
3.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou, Guangdong, Peoples R China
4.Univ Florida GCREC, Wimauma, FL USA
5.Univ Arizona, Sch Plant Sci, Tucson, AZ USA
6.Univ Arizona, Inst BIO5, Tucson, AZ USA
7.Colorado State Univ, Ft Collins, CO 80523 USA
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期： 2011 年 122 卷 2 期
页码：
收录情况： SCI
摘要： SHZ-2 is an indica rice cultivar that exhibits broad-spectrum resistance to rice blast; it is widely used as a resistance donor in breeding programs. To dissect the QTL responsible for broad-spectrum blast resistance, we crossed SHZ-2 to TXZ-13, a blast susceptible indica variety, to produce 244 BC(4)F(3) lines. These lines were evaluated for blast resistance in greenhouse and field conditions. Chromosomal introgressions from SHZ-2 into the TXZ-13 genome were identified using a single feature polymorphism microarray, SSR markers and gene-specific primers. Segregation analysis of the BC(4)F(3) population indicated that three regions on chromosomes 2, 6, and 9, designated as qBR2.1, qBR6.1, and qBR9.1, respectively, was associated with blast resistance and contributed 16.2, 14.9, and 22.3%, respectively, to the phenotypic variance of diseased leaf area (DLA). We further narrowed the three QTL regions using pairs of sister lines extracted from heterogeneous inbred families (HIF). Pairwise comparison of these lines enabled the determination of the relative contributions of individual QTL. The qBR9.1 conferred strong resistance, whereas qBR2.1 or qBR6.1 individually did not reduce disease under field conditions. However, when qBR2.1 and qBR6.1 were combined, they reduced disease by 19.5%, suggesting that small effect QTLs contribute to reduction of epidemics. The qBR6.1 and qBR9.1 regions contain nucleotide-binding sites and leucine rich repeats (NBS-LRR) sequences, whereas the qBR2.1 did not. In the qBR6.1 region, the patterns of expression of adjacent NBS-LRR genes were consistent in backcross generations and correlated with blast resistance, supporting the hypothesis that multiple resistance genes within a QTL region can contribute to non-race-specific quantitative resistance.
- 相关文献
作者其他论文 更多>>
-
Pangenome-Wide Association Study and Transcriptome Analysis Reveal a Novel QTL and Candidate Genes Controlling both Panicle and Leaf Blast Resistance in Rice
作者：Wang, Jian;Hu, Haifei;Zhang, Shaohong;Yang, Wu;Dong, Jingfang;Yang, Tifeng;Ma, Yamei;Zhou, Lian;Chen, Jiansong;Nie, Shuai;Liu, Chuanguang;Liu, Bin;Zhao, Junliang;Wang, Jian;Hu, Haifei;Zhang, Shaohong;Yang, Wu;Dong, Jingfang;Yang, Tifeng;Ma, Yamei;Zhou, Lian;Chen, Jiansong;Nie, Shuai;Liu, Chuanguang;Liu, Bin;Zhao, Junliang;Wang, Jian;Hu, Haifei;Zhang, Shaohong;Yang, Wu;Dong, Jingfang;Yang, Tifeng;Ma, Yamei;Zhou, Lian;Chen, Jiansong;Nie, Shuai;Liu, Chuanguang;Liu, Bin;Zhao, Junliang;Wang, Jian;Hu, Haifei;Zhang, Shaohong;Yang, Wu;Dong, Jingfang;Yang, Tifeng;Ma, Yamei;Zhou, Lian;Chen, Jiansong;Nie, Shuai;Liu, Chuanguang;Liu, Bin;Zhao, Junliang;Zhu, Xiaoyuan;Yang, Jianyuan;Zhu, Xiaoyuan;Yang, Jianyuan;Jiang, Xianya;Ning, Yuese
关键词：Rice; Blast resistance; Pangenome-wide association study; Transcriptomic analysis; Candidate genes
-
GWAS and Transcriptomic Analysis Identify OsRING315 as a New Candidate Gene Controlling Amylose Content and Gel Consistency in Rice
作者：Nie, Shuai;Chen, Luo;Zheng, Minhua;Dong, Jingfang;Ma, Yamei;Zhou, Lian;Wang, Jian;Chen, Jiansong;Hu, Haifei;Yang, Tifeng;Zhao, Junliang;Zhang, Shaohong;Yang, Wu
关键词：Rice; Cooking quality; Genome-wide association study; Quantitative trait loci (QTL); Candidate gene
-
The Function of SD1 on Shoot Length and its Pyramiding Effect on Shoot Length and Plant Height in Rice (Oryza sativa L.)
作者：Dong, Jingfang;Ma, Yamei;Hu, Haifei;Wang, Jian;Yang, Wu;Fu, Hua;Zhang, Longting;Chen, Jiansong;Zhou, Lian;Li, Wenhui;Nie, Shuai;Zhao, Junliang;Liu, Bin;Yang, Tifeng;Zhang, Shaohong;Zhang, Longting;Liu, Ziqiang
关键词：Shoot Length; Plant Height; Causal gene; Allele Mining; Pyramiding Effect; Rice
-
A Novel Function of GW5 on Controlling the Early Growth Vigor and its Haplotype Effect on Shoot Dry Weight and Grain Size in Rice (Oryza sativa L.)
作者：Yang, Tifeng;Dong, Jingfang;Xiong, Xijuan;Zhang, Longting;Wang, Jian;Hu, Haifei;Zhou, Lian;Yang, Wu;Ma, Yamei;Fu, Hua;Chen, Jiansong;Li, Wenhui;Nie, Shuai;Liu, Bin;Wang, Feng;Zhao, Junliang;Zhang, Shaohong;Xiong, Xijuan;Zhang, Longting;Liu, Ziqiang
关键词：Genome-wide association analysis (GWAS); Quantitative trait loci (QTL); Shoot dry weight; Causal gene; Rice
-
A pangenome analysis pipeline provides insights into functional gene identification in rice
作者：Wang, Jian;Yang, Wu;Zhang, Shaohong;Hu, Haifei;Dong, Jingfang;Chen, Luo;Ma, Yamei;Yang, Tifeng;Zhou, Lian;Chen, Jiansong;Liu, Bin;Zhao, Junliang;Wang, Jian;Yang, Wu;Zhang, Shaohong;Hu, Haifei;Dong, Jingfang;Chen, Luo;Ma, Yamei;Yang, Tifeng;Zhou, Lian;Chen, Jiansong;Liu, Bin;Zhao, Junliang;Wang, Jian;Yang, Wu;Zhang, Shaohong;Hu, Haifei;Dong, Jingfang;Chen, Luo;Ma, Yamei;Yang, Tifeng;Zhou, Lian;Chen, Jiansong;Liu, Bin;Zhao, Junliang;Hu, Haifei;Li, Chengdao;Yuan, Yuxuan;Yuan, Yuxuan;Edwards, David;Edwards, David
关键词：Pangenome; Presence; absence variation; Genomic diversity; PAV-based GWAS
-
Genome-wide association mapping combined with gene-based haplotype analysis identify a novel gene for shoot length in rice (Oryza sativa L.)
作者：Yang, Tifeng;Dong, Jingfang;Zhao, Junliang;Zhang, Longting;Zhou, Lian;Yang, Wu;Ma, Yamei;Wang, Jian;Fu, Hua;Chen, Jiansong;Li, Wenhui;Hu, Haifei;Liu, Bin;Zhang, Shaohong;Zhang, Longting;Liu, Ziqiang;Jiang, Xianya
关键词：
-
Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice
作者：Wang, Daiqi;Xu, Xiaomei;Wang, Man;Wang, Yahuan;Chen, Hong;Ping, Fei;Zhong, Huanhuan;Mu, Zhengkun;Xie, Wantong;Li, Xiangyu;Feng, Jingbin;Zhang, Milan;Zhang, Guiquan;Liu, Ziqiang;Wang, Daiqi;Wang, Man;Ping, Fei;Mu, Zhengkun;Xie, Wantong;Li, Xiangyu;Feng, Jingbin;Zhang, Milan;Zhang, Guiquan;Liu, Ziqiang;Wang, Daiqi;Ruan, Ying;Liu, Chunlin;Wang, Daiqi;Liu, Chunlin;Wang, Hongru;Fan, Zhilan;Yang, Tifeng;Zhao, Junliang;Liu, Bin;Ruan, Ying
关键词：
