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Polymorphisms associated with egg number at 300 days of age in chickens

文献类型: 外文期刊

作者: Xu, H. P. 1 ; Zeng, H. 1 ; Zhang, D. X. 1 ; Jia, X. L. 1 ; Luo, C. L. 2 ; Fang, M. X. 3 ; Nie, Q. H. 1 ; Zhang, X. Q. 1 ;

作者机构: 1.S China Agr Univ, Dept Anim Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Guangdong, Peoples R China

3.Jinan Univ, Coll Med, Dept Lab Anim Sci, Guangzhou, Guangdong, Peoples R China

关键词: Association analysis;Chicken;Polymorphism;QTL;Candidate gene;Egg number at 300 days of age

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2011 年 10 卷 4 期

页码:

收录情况: SCI

摘要: We looked for variations that could be associated with chicken egg number at 300 days of age (EN300) in seven genes of the hypothalamic-pituitary-gonadal axis, including gonadotrophin-releasing hormone-I (GnRH-I), GnRH receptor (GnRHR), neuropeptide Y (NPY), dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP), VIP receptor-1 (VIPR-1), prolactin (PRL), and the QTL region between 87 and 105 cM of the Z chromosome. Ten mutations in the seven genes were chosen to do marker-trait association analyses in a population comprising 1310 chickens, which were obtained from a company located in Guangdong Province of China. The C1704887T of VIPR-1 was found to have a highly significant association with EN300. The T5841629C of DRD2 and the C1715301T of VIPR-1 were significantly associated with EN300. A highly significant association was also found between the C1704887T-C1715301T haplotypes of VIPR-1 and EN300. H1H3 had the highest EN300. Four PCR-RFLP variations in the candidate QTL region were selected to investigate their genetic effects on EN300. The haplotypes of T32742468C-G32742603A in this region showed a highly significant association with EN300. Bioinformatics analyses showed that both T32742468C and G32742603A were located in intron 1 of the SH3-domain GRB2-like 2 (SH3GL2) gene. We conclude that five SNPs, including C1704887T and C1715301T of VIPR-1, T5841629C of DRD2, and T32742468C and G32742603A of SH3GL2, would be useful as markers for breeding to increase chicken EN300.

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