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CR-M: an Important Nucleotide Acid Sequence to Distinguish Banana Bunchy Top Virus Isolates from Two Groups

文献类型: 外文期刊

作者: Huang, Y. H. 1 ; Zeng, J. W. 1 ; Wu, Y. L. 1 ; Xia, R. 1 ; Yi, G. J. 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Fruit Tree Res Inst, Guangzhou, Guangdong, Peoples R China

期刊名称:INTERNATIONAL ISHS-PROMUSA SYMPOSIUM ON GLOBAL PERSPECTIVES ON ASIAN CHALLENGES

ISSN: 0567-7572

年卷期: 2011 年 897 卷

页码:

收录情况: SCI

摘要: A new full nucleotide sequence of BBTV 6 was cloned and analysed. The results showed it contained two main characteristic sequences: the CR-SL (Stem-loop common regions) and the CR-M (Major common regions). The phylogenetic tree, using amino acid sequences of BBTV 6, suggested that BBTV contained two groups. The nucleotide and amino acid sequences of six BBTV components were aligned and compared. The highest identities of nucleotide and amino acid sequences within each component were 96.10 and 98.27%, and between the six components 70.78 and 56.24%, respectively. All nucleotides sequences of the six BBTV components contained a major open reading frame (ORF), except BBTV 2. All six components also incorporated characteristic sequences such as CR-SL, CR-M, potential TATA box and potential polyadenylation signal. However, these characteristic sequences were somewhat different in different components. Each CR-M of all six BBTV components contained a 16-nucleotide GC-rich region. Furthermore, an almost complete 16-nucleotide direct repeat (ATACAAc/gACa/gCTATGA) was located from 4 to 19 nt and from 21 to 36 nt in isolates from the South Pacific group, but not in isolations from the Asian group. The phylogenetic trees using full nucleotide sequences and amino acid sequences of six BBTV components indicated six groups corresponding to each BBTV component. The CR-M nucleotide sequence indicated two groups according to the place of origin. In addition, the secondary structure and some physicochemical properties of proteins of the six BBTV components were predicted, showing significant differences.

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