Monoclonal antibodies against the nucleocapsid proteins of henipaviruses: production, epitope mapping and application in immunohistochemistry
文献类型： 外文期刊
作者： Xiao, C. 1 ; Liu, Y. 1 ; Jiang, Y. 1 ; Magoffin, D. E. 2 ; Guo, H. 1 ; Xuan, H. 4 ; Wang, G. 4 ; Wang, L. -F. 2 ; Tu, C. 1 ;
作者机构： 1.Acad Mil Med Sci, Inst Vet Sci, Changchun 130062, Peoples R China
2.Australian Biosecurity Cooperat Res Ctr Emerging, Geelong, Vic, Australia
3.CSIRO, Livestock Ind, Australian Anim Hlth Lab, Geelong, Vic, Australia
4.Guangdong Acad Agr Sci, Vet Res Inst, Guangzhou, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2008 年 153 卷 2 期
页码：
收录情况： SCI
摘要： Four monoclonal antibodies (mAbs) were generated by immunizing BALB/C mice with recombinant nucleocapsid protein (N) of Nipah virus (NiV) and Hendra virus (HeV) expressed in E. coli. Two mAbs each were obtained for the HeV N and NiV N, respectively. All four mAbs displayed specific reactivity with the recombinant N proteins of both viruses by western blot, which was further confirmed by immunofluorescent antibody assay using fixed insect cells infected with recombinant baculoviruses expressing either the HeV or NiV N protein. Epitope mapping using a 12-mer random peptide phage display library revealed two linear antigenic sites of the henipavirus N proteins, KLxR (aa 17-20) and FKREM (aa 446-450), which have not been reported previously. Two of the mAbs were able to specifically recognize HeV antigens by immunohistochemical staining of lung tissue sections of a horse experimentally infected with HeV. These reagents will be a useful addition to the collection of tools essential for further research and improvement in diagnosis of henipaviruses.
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