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The relationships among Cymbidium sinense cultivars based on RAPD analysis

文献类型： 外文期刊

作者： Zhu, Genfa 1 ; Li, Dongmei 1 ; Ye, Qingsheng 2 ; Guo, Zhenfei 3 ;

作者机构： 1.Guangdong Acad Agr Sci, Floriculture Res Inst, Guangzhou 510640, Guangdong, Peoples R China

2.South Normal Univ, Coll Life Sci, Guangdong Key Lab Biotechnol Plant Dev, Guangzhou 510640, Guangdong, Peoples R China

3.South China Agr Univ, Coll Life Sci, Guangzhou 510640, Guangdong, Peoples R China

关键词： Cymbidium sinense;RAPD;genetic relationship;cultivar identification

期刊名称：PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON ORNAMENTALS, NOW!

ISSN： 0567-7572

年卷期： 2008 年 766 期

页码：

收录情况： SCI

摘要： Cymbidium sinense, a popular ornamental, has a long history of cultivation in China. Many cultivars have been selected and used; however, their genetic background was unknown. The objective of this study was to determine their genetic relationships using randomly amplified polymorphic DNA (RAPD) markers. Thirty-two cultivars which mainly originated from Guangdong, Fujian, and Taiwan provinces of China were screened for polymorphic RAPD markers. Genomic DNA was extracted from green leaves of these cultivars. Sixteen random decamer primers were selected for PCR amplification. Among the 195 DNA bands obtained, 179 bands were polymorphic. The average number of DNA bands amplified by each primer was 12.2. All cultivars could be identified based on RAPD markers when a number of primers were considered. Based on dice coefficients, genetic similarity among the cultivars ranged from 0.600 to 0.870. The cultivars can be divided into two clusters based on the UPGMA dendrogram, and this division is in consistence with the morphological classification based on flower color. Cultivars originated from the same area were clustered together based on the principal coordinates analysis. It is suggested that RAPD markers may be used for cultivar identification and relationship analysis in C. sinense.

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