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Molecular Mapping of Restriction-Site Associated DNA Markers in Allotetraploid Upland Cotton

文献类型： 外文期刊

作者： Wang, Yangkun 1 ; Ning, Zhiyuan 1 ; Hu, Yan 1 ; Chen, Jiedan 1 ; Zhao, Rui 1 ; Chen, Hong; Ai, Nijiang; Guo, Wangzh 1 ;

作者机构： 1.Nanjing Agr Univ, Minist Educ, Natl Key Lab Crop Genet & Germplasm Enhancement, Cotton Hybrid R&D Engn Ctr, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Minist Educ, Natl Key Lab Crop Genet & Germplasm Enhancement, Cotton Hybrid R&D Engn Ctr, Nanjing 210095,

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation sequencing (NGS) platform has greatly expedited SNP discovery in crops with highly repetitive and complex genomes. Here we applied restriction-site associated DNA (RAD) sequencing technology for de novo SNP discovery in allotetraploid cotton. We identified 21,109 SNPs between the two parents and used these for genotyping of 161 recombinant inbred lines (RILs). Finally, a high dense linkage map comprising 4,153 loci over 3500-cM was developed based on the previous result. Using this map quantitative trait locus (QTLs) conferring fiber strength and Verticillium Wilt (VW) resistance were mapped to a more accurate region in comparison to the 1576-cM interval determined using the simple sequence repeat (SSR) genetic map. This suggests that the newly constructed map has more power and resolution than the previous SSR map. It will pave the way for the rapid identification of the marker-assisted selection in cotton breeding and cloning of QTL of interest traits.

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