您好，欢迎访问新疆农垦科学院 机构知识库！
新疆农垦科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The effect of VEGF on the temporal-spatial change of alpha-tubulin and cortical granules of ovine oocytes matured in vitro

文献类型： 外文期刊

作者： Cao, Xin 1 ; Zhou, Ping 3 ; Luo, Hailing 1 ; Zhao, Youzhang 2 ; Shi, Guoqing 3 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

2.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, Shihezi 832000, Xinjiang, Peoples R China

关键词： VEGF;alpha-Tubulin;Cortical granules;Nuclear configuration;Ovine oocyte

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2009 年 113 卷 1-4 期

页码：

收录情况： SCI

摘要： The effects of vascular endothelial growth factor (VEGF) on the temporal-spatial change of alpha-tubulin and cortical granules (CGs) in ovine oocytes matured in vitro were studied using human recombinant VEGF(165) at 5 ng/ml in maturation media. Immunofluorescence, confocal microscopy and orcein staining were used to evaluate cell cycle-dependent modifications in nuclear configuration, quality of the metaphase II oocytes, microtubule organizing centers (MTOCs) translocation, temporal and spatial redistribution of alpha-tubulin and CGs in ovine oocytes undergoing in vitro maturation. The percentage of oocytes that reached metaphase II (M-II) in the VEGF-treated and the control groups were 87.08% and 80.03% (P = 0.077) at 18 h and 87.42% and 83.89% (P = 0.28) at 24 h, respectively. The percentages of oocytes displaying a normal distribution of alpha-tubulin and chromosomes in M-II increased significantly (P = 0.015) in the VEGF group (77.50%) compared to the control (62.60%). The percentage of oocytes with CGs transfering completely in cortex was significantly higher (P = 0.002) in the VEGF group (79.24%) than in the control group (60.97%). VEGF promoted the MTOCs domains to disappear from the cortex and stimulated assembly of alpha-tubulin around chromosome domains when germinal vesicle breakdown (GVBD) was commencing. In conclusion, VEGF may improve the quality of nuclear and cytoplasmic maturation of ovine oocytes in vitro by its effect on temporal and spatial translocation or redistribution of alpha-tubulin and CGs and on the normal distribution of nuclear configuration. (c) 2008 Elsevier B.V. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农垦科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台