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Illumina-based analysis of bacterial diversity related to halophytes Salicornia europaea and Sueada aralocaspica

文献类型： 外文期刊

作者： Sh, Ying-wu 1 ; Lou, Kai 1 ; Li, Chun 1 ; Wang, Lei 2 ; Zhao, Zhen-yong 2 ; Zhao, Shuai 2 ; Tian, Chang-yan 2 ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Microbiol, Xinjiang Lab Special Environm Microbiol, Urumqi 830091, Xinjiang, Peoples R China

2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Xinjiang, Peoples R China

关键词： halophyte;endophytic bacteria;diversity;Illumina;16S rRNA gene

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2015 年 53 卷 10 期

页码：

收录情况： SCI

摘要： We used Illumina-based 16S rRNA V3 amplicon pyrosequencing to investigate the community structure of soil bacteria from the rhizosphere surrounding Salicornia europaea, and endophytic bacteria living in Salicornia europaea plants and Sueada aralocaspica seeds growing at the Fukang Desert Ecosystem Observation and Experimental Station (FDEOES) in Xinjiang Province, China, using an Illumina genome analyzer. A total of 89.23 M effective sequences of the 16S rRNA gene V3 region were obtained from the two halophyte species. These sequences revealed a number of operational taxonomic units (OTUs) in the halophytes. There were between 22-2,206 OTUs in the halophyte plant sample, at the 3% cutoff level, and a sequencing depth of 30,000 sequences. We identified 25 different phyla, 39 classes and 141 genera from the resulting 134,435 sequences. The most dominant phylum in all the samples was Proteobacteria (41.61%-99.26%; average, 43.30%). The other large phyla were Firmicutes (0%-7.19%; average, 1.15%), Bacteroidetes (0%-1.64%; average, 0.44%) and Actinobacteria (0%-0.46%; average, 0.24%). This result suggested that the diversity of bacteria is abundant in the rhizosphere soil, while the diversity of bacteria was poor within Salicornia europaea plant samples. To the extent of our knowledge, this study is the first to characterize and compare the endophytic bacteria found within different halophytic plant species roots using PCR-based Illumina pyrosequencing method.

  • 相关文献

[1]Analysis of Endophytic Bacterial Community Composition by 16S rDNA Clone Library in Achnatherum inebrians. Shi, Ying Wu,Zhang, Xuebing,Lou, Kai.

[2]Growth promotion effects of the endophyte Acinetobacter johnsonii strain 3-1 on sugar beet. Shi, Yingwu,Lou, Kai,Shi, Yingwu,Li, Chun. 2011

[3]Molecular characterization of the planktonic microorganisms in water of two mountain brackish lakes. Zeng, Jun,Deng, Li-Juan,Lou, Kai,Zhang, Tao,Yang, Hong-Mei,Shi, Ying-Wu,Lin, Qing,Zeng, Jun.

[4]Diversity of prokaryotes associated with soils around coal-fire gas vents in MaNasi county of Xinjiang, China. Lou, Kai,Zhang, Tao,Xu, Jianhua,Zeng, Jun. 2013

[5]Diversity and space-time dynamics of endophytic archaea from sugar beet in the north slope of Tianshan Mountain revealed by 454 pyrosequencing and T-RFLP. Shi, YingWu,TaPa, MuSi,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Sun, Jian,Zeng, Jun,Lin, Qing,Cao, ZhenHua,OuTi, KuEr,Li, YuGuo,Lou, Kai,Shi, YingWu,TaPa, MuSi,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Sun, Jian,Zeng, Jun,Lin, Qing,Cao, ZhenHua,OuTi, KuEr,Li, YuGuo,Lou, Kai.

[6]Endophytic fungal diversity and space-time dynamics in sugar beet. Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai,Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai.

[7]Distinctive Mesorhizobium populations associated with Cicer arietinum L. in alkaline soils of Xinjiang, China. Zhang, Jun Jie,Wang, En Tao,Tian, Chang Fu,Sui, Xin Hua,Chen, Wen Feng,Chen, Wen Xin,Zhang, Jun Jie,Wang, En Tao,Tian, Chang Fu,Sui, Xin Hua,Chen, Wen Feng,Chen, Wen Xin,Lou, Kai,Jin, Xiang,Mao, Pei Hong,Wang, En Tao. 2012

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