MODIFIED METHODS FOR THE ANALYSIS OF THE LIGNIN-LIKE PHENOLIC POLYMER CONTENTS OF COTTON FIBERS
文献类型： 外文期刊
作者： Hu, Wen-Ran 1 ; Fan, Ling 2 ; Tian, Xiao-Li 1 ; Xie, Li-Xia 1 ;
作者机构： 1.China Agr Univ, State Key Lab Plant Physiol & Biochem, Key Lab Crop Cultivat & Farming Syst, Ctr Crop Chem Control, Beijing 100193, Peoples R China
2.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Urumqi 830091, Peoples R China
3.Xinjiang Agr Univ, Agron Dept, Urumqi 830052, Peoples R China
关键词： lignin-like phenolic polymers;cotton fiber;thioglycolic acid (TGA);acetyl bromide (AB);Klason method
期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）
ISSN： 1018-7081
年卷期： 2015 年 25 卷 3 期
页码：
收录情况： SCI
摘要： The lignin-like phenolic polymer contents of cotton fibers are too little to be directly measured using existing methods. In this study, the thioglycolic acid (TGA), acetyl bromide (AB) and Klason methods were modified in order to determine the lignin-like phenolic polymer contents of cotton fibers. Varying sample weight, thioglycolicacidtoHCl ratios, extraction times, and degrees of acidificationto precipitate the lignin-likephenolicpolymers were used to study their effects on the TGA method. In addition, the effects of the digestive temperature, time, volume of the digestive solution, and ratio of NaOH to hydroxylamine hydrochlorideon the elimination of background absorbance in the AB method were tested. Furthermore, the effects of the drying temperature and sample weights on the measurement of lignin-like phenolic polymer contents using the Klason method were analyzed. The modified TGA, AB, and Klason methods were established based on their corresponding parameter modifications. These modified methods could be directly used to determine the contents of lignin-like phenolicpolymers in cotton fibers in different ways.
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